Abstract In this study, mixed culture obtained from waste water was subjected to enzyme extraction which was used with carboxymethyl cellulose (CMC) in microbial fuel cell (MFCs) for electricity generation. Higher current density of the mixed culture enriched on electrode surface in the presence of dialyzed enzyme was 360 μA cm−2 than without enzyme (307 μA cm−2). Similarly, power density of MFCs with the dialyzed enzyme (866 ± 16.6 mW m−2) was also higher than that without enzyme (737 ± 2.0 mW m−2). Sequencing and relative abundance analysis of community diversity in the enriched exoelectrogenic mixed culture biofilms in presence and absence of dialyzed enzyme added system showed Tolumonas as ratio of 50%, being much higher than that in the enriched community without adding enzyme (less than 10%). Among them three strains of n1, n2 and n3 were isolated and evaluated for their cellulase activity with 0.67 IU mL−1 min−1, 0.46 IU mL−1 min−1 and 0.46 IU mL−1 min−1, respectively. These results demonstrated that microbial crude enzyme subjected to partial purification had effect on bacteria community diversity composition of the mixed culture enriched on the electrode which improved the electricity generation performance of MFC by hydrolyzing CMC as carbon source.

中文翻译：

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从纤维素分解细菌中提取纤维素酶通过降解纤维素促进生物发电

摘要 在这项研究中，从废水中获得的混合培养物经过酶提取，与羧甲基纤维素（CMC）在微生物燃料电池（MFC）中用于发电。在有透析酶的情况下，电极表面富集的混合培养物的电流密度比没有酶的情况下（307 μA cm-2）高 360 μA cm-2。类似地，具有透析酶的 MFC 的功率密度 (866 ± 16.6 mW m-2) 也高于没有酶的 MFC (737 ± 2.0 mW m-2)。存在和不存在透析酶添加系统的富集外生电混合培养生物膜中群落多样性的测序和相对丰度分析表明，Tolumonas 的比例为 50%，远高于未添加酶的富集群落（小于 10%） . 其中3株n1，分离 n2 和 n3 并评估它们的纤维素酶活性，分别为 0.67 IU mL-1 min-1、0.46 IU mL-1 min-1 和 0.46 IU mL-1 min-1。这些结果表明，经过部分纯化的微生物粗酶对电极上富集的混合培养物的细菌群落多样性组成有影响，通过水解作为碳源的CMC提高了MFC的发电性能。