Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 °C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.

功能性产品以及天然产品的制备需要无毒但有效的提取技术。在这项研究中，亚临界水提取被用于提取不同的Aronia部分，以探索其潜力。茎，叶和浆果阿尼亚melanocarpa在以下条件下提取：温度130°C；压力35 bar; 时间20分钟。用常规的分光光度法评估所产生的提取物中的总酚和类黄酮含量。此外，还通过带有二极管阵列检测（HPLC-DAD）的高效液相色谱法对主要酚类化合物进行了鉴定和定量。提取物的生物潜力通过确定其抗氧化剂（DPPH，ABTS和脂质过氧化测定），抗菌，酶抑制（胆碱酯酶和弹性蛋白酶）和细胞毒性作用（HeLa，A-549，LS-174T，MRC-5细胞系）进行评估）。结果表明，与茎相比，叶和浆果提取物表现出更强的抗氧化作用。浆果提取物中还发现最强的胆碱酯酶和弹性蛋白酶抑制活性。同样，从叶子和果实中提取的提取物对测试的细胞系显示出相当大的细胞毒性作用。也观察到中等的抗菌作用。证实了三个Aronia部分的生物潜力，可以为开发新设计的功能产品找到新的路线图。