The detection of hepatitis B surface antigen (HBsAg), a coat protein secreted by hepatitis B virus, is a common method for confirming infection in early diagnosis. A new composite of graphene oxide‐ferrocene‐chitosan (GO‐Fc‐CS) was synthesized and used to develop an electrochemical immunosensor with a GO‐Fc‐CS/Au‐nanoparticle layer film for HBsAg detection. The modified layer film exhibited not only enhanced electron conductivity, but also strong reversible redox signal for current changes, excellent biocompatibility, and good film‐forming ability for binding a large number of antibodies. The immunosensor exhibits a wide linear range from 0.05 to 150 ng/mL and detection limit of 0.01 ng/mL for HBsAg. The excellent performance in HBsAg detection suggests that the proposed immunosensor has potential application in clinical diagnosis.

中文翻译：

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石墨烯-二茂铁-二茂铁基纳米复合材料的电化学免疫传感器用于乙型肝炎表面抗原检测

乙型肝炎表面抗原（HBsAg）（一种由乙型肝炎病毒分泌的外壳蛋白）的检测是在早期诊断中确认感染的常用方法。合成了一种新的氧化石墨烯-二茂铁-壳聚糖复合物（GO-Fc-CS），并用于开发具有GO-Fc-CS /金纳米颗粒层膜的HBsAg检测的电化学免疫传感器。修饰的层膜不仅显示出增强的电子传导性，而且还显示出针对电流变化的强可逆氧化还原信号，出色的生物相容性和良好的成膜能力，可与大量抗体结合。免疫传感器的线性范围从0.05到150 ng / mL，HBsAg的检出限为0.01 ng / mL。HBsAg检测的优异性能表明，所提出的免疫传感器在临床诊断中具有潜在的应用前景。