Size, Shape, and Protein Corona Determine Cellular Uptake and Removal Mechanisms of Gold Nanoparticles,Small

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Size, Shape, and Protein Corona Determine Cellular Uptake and Removal Mechanisms of Gold Nanoparticles
Small ( IF 13.0 ) Pub Date : 2018-09-21 , DOI: 10.1002/smll.201801451
Lin Ding ₁ , Chenjie Yao _{1,

2} , Xiaofeng Yin ₁ , Chenchen Li ₁ , Yanan Huang ₁ , Min Wu ₁ , Bin Wang ₁ , Xiaoya Guo ₁ , Yanli Wang _{1,

2} , Minghong Wu _{1,

3}

Affiliation

Size, shape, and protein corona play a key role in cellular uptake and removal mechanisms of gold nanoparticles (Au NPs). The 15 nm nanoparticles (NP1), the 45 nm nanoparticles (NP2), and the rod‐shaped nanoparticles (NR) enter into cells via a receptor‐mediated endocytosis (RME) pathway. The star‐shaped nanoparticles (NS) adopt not only clathrin‐mediated, but also caveolin‐mediated endocytosis pathways. However, the 80 nm nanoparitcles (NP3) mainly enter into the cells by macropinocytosis pathway due to the big size. Furthermore, the results indicate that the presence of protein corona can change the uptake mechanisms of Au NPs. The endocytosis pathway of NP1, NP2, and NS changes from RME to macropinocytosis pathway and NR changes from RME to clathrin and caveolin‐independent pathway under the non‐fetal bovine serun (FBS)‐coated condition. Both FBS‐coated and non‐FBS‐coated of five types of Au NPs are released out through the lysosomal exocytosis pathway. The size, shape, and protein corona have an effect on the exocytosis ratio and amount, but do not change the exocytosis mechanism. The systematic study of the endocytosis and exocytosis mechanism of Au NPs with different sizes and shapes will benefit the toxicology evaluation and nanomedicine application of Au NPs.

中文翻译：

大小，形状和蛋白质电晕确定金纳米颗粒的细胞吸收和去除机理

大小，形状和蛋白质电晕在金纳米颗粒（Au NPs）的细胞摄取和清除机制中起着关键作用。15 nm纳米颗粒（NP1），45 nm纳米颗粒（NP2）和棒状纳米颗粒（NR）通过受体介导的内吞（RME）途径进入细胞。星形纳米颗粒（NS）不仅采用网格蛋白介导，而且还利用小窝蛋白介导的内吞途径。然而，由于较大的尺寸，80 nm纳米粒子（NP3）主要通过巨胞饮途径进入细胞。此外，结果表明蛋白电晕的存在可以改变金纳米颗粒的摄取机制。在非胎牛血清（FBS）包被的情况下，NP1，NP2和NS的内吞途径从RME变为巨胞吞途径，而NR则从RME变为网格蛋白和小窝蛋白独立途径。FBS包被和非FBS包被的5种Au NP都通过溶酶体胞吐途径释放出来。大小，形状和蛋白质电晕对胞吐率和胞吐率有影响，但不会改变胞吐机理。对不同大小和形状的金纳米颗粒的内吞和胞吐机制的系统研究将有利于金纳米颗粒的毒理学评价和纳米医学应用。

更新日期：2018-09-21

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