Unnatural Chemical Biology: Research-Based Laboratory Course Utilizing Genetic Code Expansion,Journal of Chemical Education

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Unnatural Chemical Biology: Research-Based Laboratory Course Utilizing Genetic Code Expansion
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2018-09-20 00:00:00 , DOI: 10.1021/acs.jchemed.8b00011
Kelsey M. Kean ₁ , Kari van Zee ₁ , Ryan A. Mehl ₁

Affiliation

The content and design scheme for a readily adaptable, research-based laboratory course in chemical biology are presented. In this course, students interrogate protein structure and function using the site-specific incorporation of noncanonical amino acids by genetic code expansion. The relatively new field of genetic code expansion enables protein engineering with a diverse array of chemical functional groups. In this quarter or semester research-based undergraduate laboratory experience, student teams design, synthesize, and evaluate the structure–function relationships of proteins containing noncanonical amino acids on the basis of a self-selected hypothesis and then communicate their results in a formal manuscript and research presentation. The flexibility and versatility of genetic code expansion and this course structure empower students to engineer novel biomolecules and highlight the use of organic-chemistry principles for interrogating proteins. Generating noncanonical protein variants that have never been produced previously exposes students to novel research and trains them in essential basic biochemistry skills. This research-based undergraduate laboratory course can easily be adapted, scaled, and implemented on the basis of the interests, demographics, and resources of a given institution.

中文翻译：

非自然化学生物学：利用遗传密码扩展的基于研究的实验室课程

提出了一种内容容易，适应性强，以研究为基础的化学生物学实验课程的设计方案。在本课程中，学生通过遗传密码扩展使用非典型氨基酸的位点特异性掺入来询问蛋白质的结构和功能。相对较新的遗传密码扩展领域使蛋白质工程具有多种化学官能团成为可能。在本季度或学期基于研究的本科实验室经验中，学生团队根据自选假设设计，合成和评估包含非规范氨基酸的蛋白质的结构-功能关系，然后将其结果以正式手稿和研究报告。遗传密码扩展的灵活性和多功能性以及该课程的结构使学生能够设计出新颖的生物分子，并着重介绍了使用有机化学原理来探究蛋白质的方法。生成以前从未生产过的非规范蛋白质变体，使学生可以进行新颖的研究，并训练他们基本的基本生物化学技能。基于研究的本科实验室课程可以根据给定机构的兴趣，人口统计学和资源轻松进行调整，扩展和实施。

更新日期：2018-09-20

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