The RAW264.7 cell model was employed to screen immunomodulatory selenium-containing peptides from selenium-enriched rice protein hydrolysates (SPHs). Moreover, the selenium-containing peptides of high-activity protein hydrolysates were purified by Sephadex G-25, and identified by reversed phase ultra performance liquid chromatography coupled with triple quadrupole time-of-flight mass spectrometry. The results showed that 25 peptide sequences containing selenomethionine (SeMet) information above 90% of probability confidence were found in a fraction of alcalase hydrolysates. SeMDPGQQ and TSeMMM of 100% probability confidence were speculated as two novel selenium-containing peptide sequences. The artificially synthesized peptide TSeMMM was subsequently verified by an excellent immunomodulatory activity at a concentration of 80 μg/mL. In conclusion, the immunomodulatory activity of SPHs was correlated to SeMet sequence in the structure of selenium-containing peptides, and TSeMMM with a stronger immunomodulatory activity demonstrated potential as functional food additives for improving human health.

RAW264.7细胞模型用于从富硒大米蛋白水解物（SPHs）中筛选免疫调节的含硒肽。此外，通过Sephadex G-25纯化高活性蛋白水解产物的含硒肽，并通过反相超高效液相色谱结合三重四极杆飞行时间质谱进行鉴定。结果表明，在一部分碱性蛋白酶水解产物中发现了25个含有硒甲硫氨酸（SeMet）信息的肽序列，其概率置信度超过90％。推测置信度为100％的SeMDPGQQ和TSeMMM是两个新颖的含硒肽序列。随后通过浓度为80μg/ mL的出色的免疫调节活性验证了人工合成的肽TSeMMM。综上所述，