Electrochemical Monitoring of Saos‐2 Cell Differentiation on Pyrolytic Carbon Electrodes,Electroanalysis

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Electrochemical Monitoring of Saos‐2 Cell Differentiation on Pyrolytic Carbon Electrodes
Electroanalysis ( IF 2.7 ) Pub Date : 2018-09-20 , DOI: 10.1002/elan.201800429
Yasmin Mohamed Hassan ₁ , Leonardo Massa ₁ , Claudia Caviglia ₁ , Stephan Sylvest Keller ₁

Affiliation

In this study we establish an electrochemical platform based on two dimensional (2D) pyrolytic carbon electrodes for in vitro analysis of osteoblast differentiation. Electrochemical impedance spectroscopy (EIS) was used to monitor cell adhesion and proliferation, while an electrochemical assay based on square wave voltammetry (SWV) was applied to measure the activity of the differentiation marker alkaline phosphatase (ALP). 2D pyrolytic carbon electrodes were fabricated and used to monitor Saos‐2 cell differentiation for a period of up to 21 days. With this method it was possible to detect a faster increase of ALP activity for cells cultured in medium supplemented with differentiation factors compared to cells cultured in growth medium. This was confirmed by the results obtained with Alizarin Red staining, showing that cells subjected to osteogenic medium went through the entire differentiation process, from proliferation to mineralization. Finally, for the first time, real‐time monitoring of ALP activity combined with continuous EIS monitoring of the same cell culture was achieved using the pyrolytic carbon electrodes.

中文翻译：

热解碳电极上Saos-2细胞分化的电化学监测

在这项研究中，我们建立了一个基于二维（2D）热解碳电极的体外电化学平台成骨细胞分化的分析。电化学阻抗光谱法（EIS）用于监测细胞粘附和增殖，而基于方波伏安法（SWV）的电化学分析法则用于测量分化标记碱性磷酸酶（ALP）的活性。二维热解碳电极被制造出来，并用于监测Saos-2细胞分化长达21天。与在生长培养基中培养的细胞相比，使用这种方法可以检测到在补充有分化因子的培养基中培养的细胞的ALP活性更快地增加。茜素红染色得到的结果证实了这一点，表明接受成骨培养基的细胞经历了从增殖到矿化的整个分化过程。最后，第一次

更新日期：2018-09-20

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