There are some genes associated to the risk of chronic diseases that present potential nutrigenetic response, such as the human manganese-dependent superoxide dismutase gene (Val16Ala-SOD2, rs4880) for which homozygous genotypes (VV and AA) are associated with higher basal superoxide (S) and hydrogen peroxide (HP) levels, respectively. It is possible that the VV- and AA-imbalance could be attenuated by selenium(Se)-rich foods such as Brazil nut (BN). To test this hypothesis, we conducted an in vitro protocol triggering a chemical S-HP imbalance by exposure of dermal fibroblast cells (HFF-1) to paraquat, which generates high S levels (VV-like treatment) and porphyrin (MnTBAP), which generates high HP levels (AA-like treatment). Modulation of cell growth and pro-oxidative and antioxidant markers were evaluated. BN aqueous extract (BNAE) most effective concentration which increased cell growth and decreased oxidative metabolism indicators of imbalanced cells was 75 ng Se/mL. However, this effect was not directly affected by the S-HP imbalance: in AA-SOD2-like cells, thioredoxin reductase (TrxR-1) gene was upregulated and in VV-SOD2-like cells an upregulation of glutathione peroxidase (GPx-1) gene expression was observed, however, this regulation occured in a homeostatic manner. These results suggest that BNAE was able to minimize negative effects in both directions of the S-HP imbalance, by modulation of different oxidative-metabolic pathways.

有一些与慢性疾病风险相关的基因具有潜在的营养遗传响应，例如人类锰依赖性超氧化物歧化酶基因（Val16Ala-SOD2，rs4880），其纯合基因型（VV和AA）与较高的基础超氧化物血症（ S）和过氧化氢（HP）的水平。富含硒（Se）的食物，例如巴西坚果（BN），可能会降低VV和AA的不平衡。为了验证该假设，我们进行了体外皮肤成纤维细胞（HFF-1）接触百草枯会触发化学S-HP不平衡，百草枯会产生高S水平（VV样处理），而卟啉（MnTBAP）会产生高HP水平（AA样处理） 。评估了细胞生长的调节以及前氧化和抗氧化标记。BN水提取物（BNAE）能增加细胞生长并降低不平衡细胞的氧化代谢指标的最有效浓度为75 ng Se / mL。但是，这种作用不受S-HP失衡的直接影响：在AA-SOD2类细胞中，硫氧还蛋白还原酶（TrxR-1）基因上调，在VV-SOD2类细胞中，谷胱甘肽过氧化物酶（GPx-1）上调）观察到基因表达，但是这种调节以体内平衡的方式发生。