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Effects of atmospheric pressure plasma jet on the conformation and physicochemical properties of myofibrillar proteins from king prawn (Litopenaeus vannamei)
Food Chemistry ( IF 8.5 ) Pub Date : 2018-09-19 , DOI: 10.1016/j.foodchem.2018.09.113
Flora-Glad Chizoba Ekezie , Jun-Hu Cheng , Da-Wen Sun

The aim of this study was to ascertain the impact of atmospheric pressure plasma jet treatment (APPJ) as a function of treatment times (0, 2, 4, 6, 8 and 10 min) on the conformation and physicochemical properties of myofibrillar proteins (MPs) extracted from king prawn (Litopenaeus vannamei). The results showed that the pH and protein solubility were decreased after 10 min treatment of APPJ. These results were also confirmed by dynamic light scattering, which indicated an increase in mean particle diameter of MPs from 654 to 2297 nm. Complementary methodologies used to characterize the structural changes confirmed the exposure of hydrophobic groups and promotion of protein-protein interactions. These occurrences were particularly more intense at the longest treatment duration. The high energetic and oxidizing species contained in the plasma gas may have considerable implications on the physical and structural characteristics of MPs from king prawn.



中文翻译:

从王虾上肌原纤维蛋白的构象和物理化学性质大气压等离子体射流的影响(凡纳滨对虾

这项研究的目的是确定大气压血浆射流治疗(APPJ)与治疗时间(0、2、4、6、8和10分钟)的关系对肌原纤维蛋白(MPs)构象和理化性质的影响)从大虾(南美白对虾(Litopenaeus vannamei))。结果表明,在APPJ处理10分钟后,pH和蛋白质溶解度均降低。这些结果也通过动态光散射得到证实,动态光散射表明MP的平均粒径从654纳米增加到2297纳米。用于表征结构变化的补充方法学证实了疏水基团的暴露和蛋白质-蛋白质相互作用的促进。在最长的治疗时间里,这些情况尤其严重。血浆气体中所含的高能和氧化性物质可能会对大虾MP的物理和结构特征产生重大影响。

更新日期:2018-09-19
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