The best acknowledged correlate of protection from norovirus (NoV) infection is the ability of serum antibodies to block binding of NoV virus-like particles (VLPs) to histo-blood group antigens (HBGAs). We investigated mucosal NoV-specific antibody levels in adult volunteers and used saliva from a single donor to determine whether purified saliva antibodies confer blocking. NoV-specific IgG and IgA levels in saliva and plasma samples were measured against four NoV genotype VLPs. NoV-specific IgG and IgA titers in saliva and plasma samples correlated significantly. Antibodies were detected against all VLPs with the highest level of antibodies directed against ancestral GII.4 99 genotype. Affinity chromatography purified salivary IgA and IgG blocked binding of GII.4 99 VLPs to HBGAs. Saliva sampling is a non-invasive alternative to blood drawing and an excellent biological fluid to study NoV-specific immune responses. Mucosal anti-NoV antibodies block binding of NoV VLPs to HBGAs, and may therefore be protective.

预防诺如病毒（NoV）感染的最佳公认关联是血清抗体阻断NoV病毒样颗粒（VLP）与组织血型抗原（HBGA）结合的能力。我们调查了成年志愿者的黏膜NoV特异性抗体水平，并使用来自单个供体的唾液来确定纯化的唾液抗体是否具有阻断作用。针对四种NoV基因型VLP测量了唾液和血浆样品中的NoV特异性IgG和IgA水平。唾液和血浆样品中的NoV特异性IgG和IgA滴度显着相关。检测到针对所有VLP的抗体，这些抗体具有针对祖先GII.4 99基因型的最高水平的抗体。亲和色谱纯化的唾液IgA和IgG阻断了GII.4 99 VLP与HBGA的结合。唾液采样是抽血的一种非侵入性替代方法，是研究NoV特异性免疫反应的出色生物液。粘膜抗NoV抗体可阻断NoV VLP与HBGA的结合，因此可能具有保护性。