This research was conducted as factorial on the basis of randomized complete block design with three replications to evaluate the effects of salicylic acid (1 mM SA), jasmonic acid (0.5 mM JA) and SA+JA on H⁺-ATPase hydrolytic activity of tonoplast in soybean roots under 0, 4, 7 and 10 dS m⁻¹ NaCl levels. The H⁺-ATPase hydrolytic activity of tonoplast was increased under 4 dS m⁻¹, but with rising salinity up to 7 and 10 dS m⁻¹, the activity of H⁺-ATPase and ATP content were decreased in root cells. Root growth, potassium, calcium, magnesium and iron contents in plant tissues were decreased, while sodium, manganese, zinc and copper contents were increased by salinity, leading to a reduction in chlorophyll content index (CCI), relative water content (RWC), plant biomass and grain yield of soybean. Treatment of plants with SA, JA and SA+JA improved H⁺-ATPase activity and ATP content in root cells. JA treatment also reduced root growth, thereby limited sodium uptake by roots and translocation to the shoots. Foliar spray of JA only increased magnesium and iron contents in plant tissues, with no significant effect on other cations. In contrast, SA and SA+JA improved root growth and enhanced most of the cations, CCI, RWC, plant biomass and consequently grain yield under different levels of salinity. The SA+JA was a superior treatment in diminishing the harmful effects of salinity on soybean plant performance, compared with individual application of these growth regulators.

本研究在随机完全区组设计的基础上进行了三次重复的析因，以评估水杨酸（1 mM SA）、茉莉酸（0.5 mM JA）和SA+JA对液泡膜H ⁺ -ATP酶水解活性的影响在 0、4、7 和 10 dS m ^-1 NaCl 水平下的大豆根中。在4 dS m ^-1下，液泡膜的H ⁺ -ATP酶水解活性增加，但随着盐度升高至7和10 dS m ^-1 ，根细胞中H ⁺ -ATP酶活性和ATP含量降低。根系生长，植物组织中钾、钙、镁、铁含量下降，而钠、锰、锌、铜含量则因盐度升高而增加，导致叶绿素含量指数（CCI）、相对含水量（RWC）、大豆植物生物量和籽粒产量。用SA、JA和SA+JA处理植物提高了根细胞中的H ⁺ -ATP酶活性和ATP含量。 JA 处理还减少了根的生长，从而限制了根对钠的吸收以及向芽的转移。叶面喷施JA仅增加植物组织中镁、铁的含量，对其他阳离子无明显影响。相比之下，SA和SA+JA在不同盐度水平下改善了根系生长并提高了大部分阳离子、CCI、RWC、植物生物量，从而提高了谷物产量。与单独施用这些生长调节剂相比，SA+JA 在减少盐度对大豆植株性能的有害影响方面是一种优越的处理方法。