Nitrogen Feeding Strategies and Metabolomic Analysis To Alleviate High-Nitrogen Inhibition on Docosahexaenoic Acid Production in Crypthecodinium cohnii,Journal of Agricultural and Food Chemistry

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Nitrogen Feeding Strategies and Metabolomic Analysis To Alleviate High-Nitrogen Inhibition on Docosahexaenoic Acid Production in Crypthecodinium cohnii
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-09-18 00:00:00 , DOI: 10.1021/acs.jafc.8b03634
Liangsen Liu _{1,

2,

3} , Fangzhong Wang ₄ , Ji Yang ₄ , Xingrui Li _{1,

2,

3} , Jinyu Cui _{1,

2,

3} , Jing Liu _{1,

2,

3} , Mengliang Shi _{1,

2,

3} , Kang Wang _{1,

2,

3} , Lei Chen _{1,

2,

3} , Weiwen Zhang _{1,

2,

3,

4}

Affiliation

It is well-known that high-nitrogen content inhibits cell growth and docosahexaenoic acid (DHA) biosynthesis in heterotrophic microalgae Crypthecodinium cohnii. In this study, two nitrogen feeding strategies, pulse-feeding and continuous-feeding, were evaluated to alleviate high-nitrogen inhibition effects on C. cohnii. The results showed that continuous-feeding with a medium solution containing 50% (w/v) yeast extract at 2.1 mL/h during 12–96 h was the optimal nitrogen feeding strategy for the fermentation process, when glucose concentration was maintained at 15–27 g/L during the same period. With the optimized strategy, 71.2 g/L of dry cell weight and DHA productivity of 57.1 mg/L/h were achieved. In addition, metabolomic analysis was applied to determine the metabolic changes during different nitrogen feeding conditions, and the changes in amino acids, polysaccharides, purines, and pentose phosphate pathway were observed, providing valuable metabolite-level information for exploring the mechanism of the high-nitrogen inhibition effect and further improving DHA productivity in C. cohnii.

中文翻译：

缓和Crypthecodinium cohnii中二十二碳六烯酸生产的高氮抑制的供氮策略和代谢组学分析

众所周知，高氮含量会抑制异养微藻Crypthecodinium cohnii中的细胞生长和二十二碳六烯酸（DHA）生物合成。在这项研究中，评估了两种氮供料策略，即脉冲供料和连续供料，以减轻对C. cohnii的高氮抑制作用。结果表明，连续饲喂含50％（w / v）当葡萄糖浓度在同一时期保持在15-27 g / L时，在12-96 h期间以2.1 mL / h的酵母提取物是发酵过程的最佳氮补料策略。通过优化的策略，可以达到71.2 g / L的干细胞重量和57.1 mg / L / h的DHA生产率。此外，通过代谢组学分析确定了在不同氮素饲喂条件下的代谢变化，并观察了氨基酸，多糖，嘌呤和磷酸戊糖途径的变化，为探索高氮代谢的机理提供了有价值的代谢物水平信息。氮的抑制作用和进一步提高了C. cohnii的DHA生产率。

更新日期：2018-09-18

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