Ajwa fruits are among the most nutritious and economically valuable fruits. In this study, the effect of different solvents on the extraction of polyphenols was investigated. Aqueous acetone (50%) extract contained the highest content of polyphenols and flavonoids. Filtration of the extract through a Diaion HP-20 column led to a polyphenols-enriched fraction (Fr 1) that had the highest antioxidant activity as measured with ORAC and DPPH assays. Metabolic profiling of the different extracts using HPLC-ESI-QTOF-MS-MS led to the tentative identification of 33 secondary metabolites: 3 phenolic acids, 19 flavonoid glycosides including 4 sulfated derivatives, 2 lignans, and 9 fatty acids. Fr 1 showed a promising hepatoprotective activity when tested in-vitro utilizing HepG2 cell lines and in-vivo against thioacetamide-induced liver fibrosis in mice. Ingestion of Fr 1 in the form of a microemulsion had significantly higher oral bioavailability than when ingested as a suspension in water.

Ajwa水果是最具营养和经济价值的水果之一。在这项研究中，研究了不同溶剂对多酚提取的影响。丙酮水溶液（50％）提取物中的多酚和类黄酮含量最高。通过Diaion HP-20色谱柱过滤萃取物，得到的多酚富集级分（Fr 1）具有最高的抗氧化活性，如ORAC和DPPH分析所测。使用HPLC-ESI-QTOF-MS-MS对不同提取物进行代谢谱分析，初步鉴定出33种次级代谢产物：3种酚酸，19种类黄酮糖苷，包括4种硫酸化衍生物，2种木脂素和9种脂肪酸。Fr 1在体外测试时显示出有希望的肝保护活性利用HepG2细胞系和体内抗硫代乙酰胺诱导的小鼠肝纤维化。与以水的悬浮液形式摄取时相比，以微乳状液形式摄取Fr 1具有更高的口服生物利用度。