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Asking more from metabolic oligosaccharide engineering
Chemical Science ( IF 7.6 ) Pub Date : 2018-09-18 00:00:00 , DOI: 10.1039/c8sc02241k Pierre-André Gilormini 1 , Anna R Batt 2 , Matthew R Pratt 2, 3 , Christophe Biot 1
Chemical Science ( IF 7.6 ) Pub Date : 2018-09-18 00:00:00 , DOI: 10.1039/c8sc02241k Pierre-André Gilormini 1 , Anna R Batt 2 , Matthew R Pratt 2, 3 , Christophe Biot 1
Affiliation
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Glycans form one of the four classes of biomolecules, are found in every living system and present a huge structural and functional diversity. As an illustration of this diversity, it has been reported that more than 50% of the human proteome is glycosylated and that 2% of the human genome is dedicated to glycosylation processes. Glycans are involved in many biological processes such as signalization, cell–cell or host pathogen interactions, immunity, etc. However, fundamental processes associated with glycans are not yet fully understood and the development of glycobiology is relatively recent compared to the study of genes or proteins. Approximately 25 years ago, the studies of Bertozzi's and Reutter's groups paved the way for metabolic oligosaccharide engineering (MOE), a strategy which consists in the use of modified sugar analogs which are taken up into the cells, metabolized, incorporated into glycoconjugates, and finally detected in a specific manner. This groundbreaking strategy has been widely used during the last few decades and the concomitant development of new bioorthogonal ligation reactions has allowed many advances in the field. Typically, MOE has been used to either visualize glycans or identify different classes of glycoproteins. The present review aims to highlight recent studies that lie somewhat outside of these more traditional approaches and that are pushing the boundaries of MOE applications.
中文翻译：
对代谢寡糖工程提出更多要求
聚糖是四类生物分子之一，存在于每个生命系统中，并呈现出巨大的结构和功能多样性。作为这种多样性的例证，据报道，超过 50% 的人类蛋白质组是糖基化的，并且 2% 的人类基因组专门用于糖基化过程。聚糖参与许多生物过程，例如信号传导、细胞与细胞或宿主病原体相互作用、免疫等。然而，与聚糖相关的基本过程尚未完全了解，与基因或基因的研究相比，糖生物学的发展相对较新。蛋白质。大约 25 年前，Bertozzi 和 Reutter 小组的研究为代谢寡糖工程 (MOE) 铺平了道路，该策略包括使用修饰的糖类似物，这些类似物被吸收到细胞中、代谢、掺入糖复合物中，最后以特定方式检测到。这种突破性的策略在过去几十年中得到了广泛应用，新的生物正交连接反应的随之发展使得该领域取得了许多进展。通常，MOE 用于可视化聚糖或识别不同类别的糖蛋白。本综述旨在强调最近的研究，这些研究在某种程度上超出了这些更传统的方法，并且正在突破 MOE 应用的界限。
更新日期：2018-09-18
中文翻译：
对代谢寡糖工程提出更多要求
聚糖是四类生物分子之一，存在于每个生命系统中，并呈现出巨大的结构和功能多样性。作为这种多样性的例证，据报道，超过 50% 的人类蛋白质组是糖基化的，并且 2% 的人类基因组专门用于糖基化过程。聚糖参与许多生物过程，例如信号传导、细胞与细胞或宿主病原体相互作用、免疫等。然而，与聚糖相关的基本过程尚未完全了解，与基因或基因的研究相比，糖生物学的发展相对较新。蛋白质。大约 25 年前，Bertozzi 和 Reutter 小组的研究为代谢寡糖工程 (MOE) 铺平了道路，该策略包括使用修饰的糖类似物，这些类似物被吸收到细胞中、代谢、掺入糖复合物中，最后以特定方式检测到。这种突破性的策略在过去几十年中得到了广泛应用，新的生物正交连接反应的随之发展使得该领域取得了许多进展。通常，MOE 用于可视化聚糖或识别不同类别的糖蛋白。本综述旨在强调最近的研究，这些研究在某种程度上超出了这些更传统的方法，并且正在突破 MOE 应用的界限。
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