In this work, a luminescent probe (RuE, the abbreviation of Ru(bpy)₂(E)Cl₂, where bpy=2,2′‐bipyridine, E=N,N′‐5,6‐(1,10‐phenanthroline)‐bis(2‐hydroxy benzaldehyde)diamine) based on the ruthenium(II) bipyridyl complex conjugated with functionalized Schiff bases, which revealed a highly selective luminescence quenching by Cu²⁺ in aqueous solution was prepared. Because of the quenching effect of copper ions, the luminescence of RuE−Cu(II) solution was weak. However, in the presence of pyrophosphate (P 2O4‐ 7, PPi), the strong binding ability of PPi to Cu²⁺ can promote the break up of RuE−Cu(II) complex and then recover the luminescence. Most of other potential interfering anions, including strong interfering substance, like phosphate, ATP, ADP and AMP can not enhance the luminescence apparently. Therefore, the RuE−Cu(II) ensemble can act as an excellently selective and sensitive turn‐on probe for the sequential determination of PPi in aqueous solution. The detection limit of the probe for PPi was 0.58 nM and the linear concentration range was 0−16 μM. In addition, the potential applicability of this probe has been demonstrated by the successful detection of PPi in human urine samples.

中文翻译：

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用于水溶液中焦磷酸盐检测的高选择性和灵敏的发光探针

在这项工作中，使用了发光探针（RuE，Ru（bpy）₂（E）Cl ₂的缩写，其中bpy = 2,2'-联吡啶，E = N，N'-5,6-（1,10-基于与功能化席夫碱共轭的钌（II）联吡啶配合物的邻二氮杂菲-双（2-羟基苯甲醛）二胺）制备了高选择性的荧光猝灭剂，该猝灭剂由水溶液中的Cu ²⁺猝灭。由于铜离子的猝灭作用，RuE-Cu（II）溶液的发光较弱。但是，在存在焦磷酸盐（P 2O4-7，PPi）的情况下，PPi与Cu ²⁺的强结合能力可以促进RuE-Cu（II）络合物的分解，然后恢复发光。大多数其他潜在的干扰阴离子，包括强干扰物质，如磷酸盐，ATP，ADP和AMP，都无法明显增强发光。因此，RuE-Cu（II）集合体可以作为顺序测定水溶液中PPi的极佳选择性和灵敏的开启探针。PPi探针的检出限为0.58 nM，线性浓度范围为0-16μM。此外，该探针的潜在适用性已通过成功检测人尿液样品中的PPi得到了证明。