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Novel bioactive peptides demonstrating anti‐dengue virus activity isolated from the Asian medicinal plant Acacia Catechu
Chemical Biology & Drug Design ( IF 3.2 ) Pub Date : 2018-10-10 , DOI: 10.1111/cbdd.13400 Aussara Panya 1, 2, 3 , Petlada Yongpitakwattana 3 , Prapaphan Budchart 3 , Nunghathai Sawasdee 3 , Sucheewin Krobthong 4 , Atchara Paemanee 4 , Sittiruk Roytrakul 4 , Siriluk Rattanabunyong 5 , Kiattawee Choowongkomon 6 , Pa-thai Yenchitsomanus 3
Chemical Biology & Drug Design ( IF 3.2 ) Pub Date : 2018-10-10 , DOI: 10.1111/cbdd.13400 Aussara Panya 1, 2, 3 , Petlada Yongpitakwattana 3 , Prapaphan Budchart 3 , Nunghathai Sawasdee 3 , Sucheewin Krobthong 4 , Atchara Paemanee 4 , Sittiruk Roytrakul 4 , Siriluk Rattanabunyong 5 , Kiattawee Choowongkomon 6 , Pa-thai Yenchitsomanus 3
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The therapeutic activities of food‐derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those traditionally prepared by water extraction for use as tea or food supplement. In this study, novel bioactive peptides were isolated from enzymatic digests of 33 Thai medicinal plants. The inhibitory activity of each against dengue virus (DENV) infection was investigated. Of 33 plants, peptides from Acacia catechu extract demonstrated the most pronounced anti‐DENV activity. Half maximal inhibitory concentration of 0.18 μg/ml effectively inhibited DENV foci formation. Treatment with 1.25 μg/ml crude peptide extract could reduce virus production less than 100‐fold with no observable cell toxicity. Peptide sequences were determined by high‐performance liquid chromatography and liquid chromatography–tandem mass spectrometry. Two bioactive peptides isolated from Acacia catechu inhibited DENV foci formation >90% at the concentration of 50 μM; therefore, they are recommended for further investigation as antiviral peptides against DENV infection.
中文翻译：
从亚洲药用植物相思树中分离出来的新型具有生物活性的肽，具有抗登革热病毒的活性
食物来源的生物活性蛋白和多肽的治疗活性在研究界引起了越来越多的关注。传统药物中使用的药用植物是生物活性蛋白质和多肽的极佳来源，尤其是传统上通过水提取制得的茶和食品补充剂。在这项研究中，新型的生物活性肽是从33种泰国药用植物的酶消化物中分离出来的。研究了每种药物对登革热病毒（DENV）感染的抑制活性。在33种植物中，相思树的肽提取物表现出最明显的抗-DENV活性。最大抑制浓度的一半为0.18μg/ ml可有效抑制DENV灶的形成。用1.25μg/ ml粗肽提取物处理可以将病毒产量降低不到100倍，并且没有可观察到的细胞毒性。通过高效液相色谱和液相色谱-串联质谱法确定肽序列。从儿茶金合欢中分离出的两种生物活性肽在50μM的浓度下可抑制DENV灶的形成> 90％。因此，建议将它们作为抗DENV感染的抗病毒肽进行进一步研究。
更新日期：2018-10-10
中文翻译：
从亚洲药用植物相思树中分离出来的新型具有生物活性的肽，具有抗登革热病毒的活性
食物来源的生物活性蛋白和多肽的治疗活性在研究界引起了越来越多的关注。传统药物中使用的药用植物是生物活性蛋白质和多肽的极佳来源，尤其是传统上通过水提取制得的茶和食品补充剂。在这项研究中，新型的生物活性肽是从33种泰国药用植物的酶消化物中分离出来的。研究了每种药物对登革热病毒（DENV）感染的抑制活性。在33种植物中，相思树的肽提取物表现出最明显的抗-DENV活性。最大抑制浓度的一半为0.18μg/ ml可有效抑制DENV灶的形成。用1.25μg/ ml粗肽提取物处理可以将病毒产量降低不到100倍，并且没有可观察到的细胞毒性。通过高效液相色谱和液相色谱-串联质谱法确定肽序列。从儿茶金合欢中分离出的两种生物活性肽在50μM的浓度下可抑制DENV灶的形成> 90％。因此，建议将它们作为抗DENV感染的抗病毒肽进行进一步研究。
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