A new generation of nanostructured glasses called mesoporous bioactive glasses (MBGs) exhibit superior surface texture, porosity and bioactive characteristics. The present study is carried out to develop and detailed characterize of ternary SiO₂-CaO-P₂O₅ MBG structure, fabricated by three different variations using different surfactants, e.g., hexadecyltrimethylammonium bromide (CTAB), poly-ethylene glycol,(PEG) and Pluronic P123. After thorough physico-chemical characterization, MBG granules were investigated for in vivo bone regeneration in animal bone defect model (rabbit) where standard S53P4 bioactive glass was used as control. All the synthesized MBG powders showed nano-range median particle size of 80–120 nm (MBG-CTAB), 50–70 nm (MBG-PEG and MBG-P123) while their specific surface area as 473.2, 52.2 and 169.3 m²/g respectively. All MBGs showed mesoporous nature corroborating transmission electron microscopy (TEM) observation as well. Bone regeneration property was measured after 45 and 90 days post-implantation at distal epiphysis of rabbit femur by radiography, histology, fluorochrome labeling, micro computed tomography (micro-CT) and vital organ histology. Results from in vivo studies indicated that the MBG materials produce minimal toxicity to the body. Furthermore, the biocompatibility and biodegradability of the implant makes them more suitable for application in bone tissue engineering. Among various implants, MBG fabricated using suitable surfactant (CTAB) shown the best result compared to other implants. Nonetheless, all the materials are suitable for application in bone tissue engineering and have potential for bone regeneration and healing.

新一代的纳米结构玻璃称为介孔生物活性玻璃（MBG），具有优异的表面纹理，孔隙率和生物活性特征。进行本研究以开发和详细表征三元SiO ₂ -CaO-P ₂ O ₅MBG结构，使用不同的表面活性剂，例如十六烷基三甲基溴化铵（CTAB），聚乙二醇，（PEG）和Pluronic P123，通过三种不同的变化制成。经过彻底的物理化学表征后，在以标准S53P4生物活性玻璃为对照的动物骨缺损模型（兔）中研究了MBG颗粒的体内骨再生。所有合成的MBG粉末均表现出80-120 nm（MBG-CTAB），50-70 nm（MBG-PEG和MBG-P123）的纳米范围中值粒径，而比表面积分别为473.2、52.2和169.3 m ²/ g。所有MBG均显示出介孔性质，也证实了透射电子显微镜（TEM）的观察。植入后45和90天，通过放射线照相，组织学，荧光染料标记，微型计算机断层扫描（micro-CT）和重要器官组织学测量兔股骨远端骨physi的骨再生特性。体内研究结果表明，MBG材料对人体产生的毒性最小。此外，植入物的生物相容性和生物降解性使其更适合在骨组织工程中应用。在各种植入物中，与其他植入物相比，使用合适的表面活性剂（CTAB）制成的MBG表现出最好的效果。但是，所有材料均适用于骨组织工程，并具有促进骨再生和愈合的潜力。