Abstract Bactericidal activity of neutral electrolyzed water (NEW) was investigated against endospore forming Bacillus cereus and Clostridium perfringens in cell suspensions and artificially inoculated onto the surface of selected fresh produce items (cherry tomato, miniature cucumber, carrot and parsley) and polypropylene cutting boards at ambient temperature (22 °C). Viable counts of survivors were determined within 0 (untreated control), 1, 3 and 5 min of treatment at ambient temperature using NEW solutions of 60 and 120 mg/L free available chlorine FAC. All treatments showed significant differences (P 0.05) after 5 min of treatment, whereas bacterial viability of the inoculated produce was reduced by 2.11–2.30 and 2.41–3.16 log10 CFU/g when NEW used at 120 mg/L FAC for B. cereus and C. perfringens, respectively. When inoculated cutting boards were sprayed with NEW at 120 mg/L FAC and after 5 min of treatment, cell viability was reduced by 2.33 and 3.06 log10 per 100 cm2 for B. cereus and C. perfringens, respectively. This study showed that NEW could be used as an effective bactericidal treatment alternative to commonly used chemical sanitizers against endospore forming bacteria.

中文翻译：

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中性电解水对细胞悬液中蜡样芽孢杆菌和产气荚膜梭菌的杀菌活性，并人工接种到选定的新鲜农产品和聚丙烯砧板表面

摘要 研究了中性电解水 (NEW) 对细胞悬浮液中形成内生孢子的蜡样芽孢杆菌和产气荚膜梭菌的杀菌活性，并人工接种到选定的新鲜农产品（樱桃番茄、微型黄瓜、胡萝卜和欧芹）和聚丙烯砧板的表面。环境温度 (22 °C)。使用 60 和 120 毫克/升游离有效氯 FAC 的新溶液在环境温度下处理 0（未处理对照）、1、3 和 5 分钟内确定存活的存活数。所有处理在处理 5 分钟后都显示出显着差异 (P 0.05)，而当 NEW 以 120 毫克/升 FAC 用于蜡状芽孢杆菌和蜡状芽孢杆菌时，接种产品的细菌活力降低了 2.11-2.30 和 2.41-3.16 log10 CFU/g。 C.产气荚膜分别。当用 120 mg/L FAC 的 NEW 喷洒接种的砧板时，处理 5 分钟后，蜡样芽孢杆菌和产气荚膜梭菌的细胞活力分别降低了 2.33 和 3.06 log10/100 cm2。这项研究表明，NEW 可作为一种有效的杀菌处理方法来替代常用的化学消毒剂，以对抗形成内生孢子的细菌。