The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on–off state of fluorophores through various chemical, photochemical, or binding–unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.

中文翻译：

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用于单分子定位显微镜的可切换荧光团

过去十年见证了超分辨率荧光显微镜方法在生物学和其他领域的使用爆炸式增长。单分子定位显微镜（SMLM）是这些方法中最广泛使用的一种，其成功在很大程度上归功于通过各种化学、光化学或结合-解除结合机制控制荧光团的开关状态的能力。我们在此提供 SMLM 中可切换荧光团的全面概述，包括对 SMLM 荧光团所有主要类别的详细回顾，我们还讨论了标记样本的策略、多通道和活细胞成像的注意事项、潜在的陷阱以及未来发展的领域。