AbstractPregnancy test strips are widely used in daily life. A commercial pregnancy test strip was modified to obtain a point-of-care device for the detection of pathogenic bacteria. Hybrid nanoflowers were prepared from concanavalin A, human chorionic gonadotropin, and Cu3(PO4)2 via a one-pot method. They were used as signaling probes in an off-the-shelf pregnancy test strip. This modified lateral flow immunoassay can detect Escherichia coli O157:H7 with a detection limit of 4 CFU·mL−1, and Salmonella typhimurium with a detection limit of 3 CFU·mL−1. Conceivably, the method has high potential as a portable and cost-effective tool for rapid determination of a wide range of analytes, especially in resource-constrained settings. Graphical abstractHybrid nanoflower loaded human chorionic gonadotropin (hCG) and concanavalin A (hCG − nanoflowers) were synthesized via a one-pot method and used as signal labels with commercial commercial-off-the-shelf pregnancy test strips to detect pathogenic bacteria targets, thus yielding an easily smartphone readout signal.

中文翻译：

---

利用刀豆蛋白 A-人绒毛膜促性腺激素-Cu3(PO4)2 混合纳米花、磁分离和智能手机读数检测病原菌的验孕试纸

摘要妊娠试纸在日常生活中应用广泛。对商业妊娠试纸进行了修改，以获得用于检测病原菌的即时护理设备。通过一锅法从伴刀豆球蛋白 A、人绒毛膜促性腺激素和 Cu3(PO4)2 制备混合纳米花。它们被用作现成的妊娠试纸中的信号探针。这种改进的侧流免疫分析法可以检测大肠杆菌 O157:H7，检测限为 4 CFU·mL-1，鼠伤寒沙门氏菌检测限为 3 CFU·mL-1。可以想象，该方法作为一种便携式且经济高效的工具具有很高的潜力，可用于快速测定范围广泛的分析物，尤其是在资源受限的环境中。