Abstract Bioactive nanostructured coating has been a key strategy to improve the cell adhesion and osseointegration of metallic implants. This study presents the electrophoretic coatings of hydroxyapatite with tunable nanocrystal morphologies. The hydroxyapatite nanocrystals were prepared with three different elongated dimensions by the hydrothermal method (∼80 nm; SHA, ∼300 nm; MHA, and ∼900 nm; LHA), which were used in combination with a fugitive biopolymer chitosan for the electrophoretic deposition on titanium. All the HA coatings were densely and homogeneously deposited and had different and unique nanotopologies and corresponding surface areas. The coating parameter significantly altered the cell behaviors including anchorage, spreading and proliferation; the cell adhesion was better on the small- and middle-sized nanocrystals (SHA, MHA > LHA), which was interestingly opposite in cell spreading (LHA > SHA, MHA), and then the cell proliferation was again up-regulated on SHA and MHA. Although more studies are needed to elucidate the biological phenomenon, the sets of results imply the importance of tailored nanocrystal morphology in the initial (adhesion and growth) cellular events.

中文翻译：

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各种纳米晶形羟基磷灰石的电泳涂层

摘要 生物活性纳米结构涂层已成为改善金属植入物细胞粘附和骨整合的关键策略。本研究展示了具有可调纳米晶体形态的羟基磷灰石电泳涂层。通过水热法（~80 nm；SHA，~300 nm；MHA 和~900 nm；LHA）制备了具有三种不同细长尺寸的羟基磷灰石纳米晶体，将其与短效生物聚合物壳聚糖结合用于电泳沉积在钛。所有 HA 涂层都密集且均匀地沉积，并具有不同且独特的纳米拓扑结构和相应的表面积。涂层参数显着改变了细胞行为，包括锚定、扩散和增殖；中小尺寸纳米晶（SHA、MHA > LHA），这在细胞扩散中是相反的（LHA > SHA，MHA），然后细胞增殖在 SHA 和 MHA 上再次上调。虽然需要更多的研究来阐明生物学现象，但这些结果暗示了定制纳米晶体形态在初始（粘附和生长）细胞事件中的重要性。