AbstractA composite consisting of graphene oxide and gold nanorods (GO-GNRs) was designed for the trace determination of hepatitis B surface antigen (HBsAg) using surface enhanced Raman spectroscopy (SERS). GO contains numerous carboxy and hydroxy groups on its surface and therefore can serve as the substrate for decoration with GNRs and for immobilizing antibody against HBsAg. The GNRs (carrying the SERS probe 2-mercaptopyridine) exhibit high SERS activity, and this improves the sensitivity of the biosensor. The antibody on the GO-GNRs binds HBsAg with high specificity, and it results in excellent selectivity. The SERS signal (measured at 1002 cm−1) increases in the 1–1000 pg·mL−1 HBsAg concentrations range, and the limit of detection is 0.05 pg·mL−1 (at an S/N ratio of 3). The immunoassay achieves the sensitive and selective determination of HBsAg in serum and expands the potential application of GO-GNR based SERS tag in clinical research. Graphical abstractA novel graphene oxide-gold nanorod (GO-GNRs) based surface-enhanced Raman scattering (SERS) tag for immunoassay was designed. It allows for sensitive and selective determination of HBsAg in serum. The method is expected to expand the potential application in the environment, in medicine and in food analysis.

中文翻译：

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在基于 SERS 的乙型肝炎表面抗原免疫测定中用金纳米棒包裹的氧化石墨烯作为标签

摘要 设计了一种由氧化石墨烯和金纳米棒 (GO-GNRs) 组成的复合材料，用于使用表面增强拉曼光谱 (SERS) 痕量测定乙型肝炎表面抗原 (HBsAg)。GO 表面含有大量羧基和羟基，因此可以作为修饰 GNR 和固定抗 HBsAg 抗体的底物。GNR（携带SERS探针2-巯基吡啶）表现出高SERS活性，这提高了生物传感器的灵敏度。GO-GNRs 上的抗体以高特异性结合 HBsAg，并具有出色的选择性。SERS 信号（在 1002 cm-1 处测量）在 1–1000 pg·mL-1 HBsAg 浓度范围内增加，检测限为 0.05 pg·mL-1（信噪比为 3）。免疫测定实现了血清中HBsAg的灵敏和选择性测定，扩大了基于GO-GNR的SERS标签在临床研究中的潜在应用。图形摘要设计了一种用于免疫测定的新型氧化石墨烯-金纳米棒 (GO-GNRs) 表面增强拉曼散射 (SERS) 标签。它允许灵敏和选择性地测定血清中的 HBsAg。该方法有望扩大在环境、医学和食品分析中的潜在应用。