In the past decade it has become clear that many microbes harbor enzymes that employ an unusual flavin cofactor, the F₄₂₀ deazaflavin cofactor. Herein we show that F₄₂₀-dependent reductases (FDRs) can successfully perform enantio-, regio- and chemoselective ene-reductions. For the first time, we have demonstrated that F₄₂₀H₂-driven reductases can be used as biocatalysts for the reduction of α,β-unsaturated ketones and aldehydes with good conversions (>99%) and excellent regioselectivities and enantiomeric excesses (>99% ee). Noteworthily, FDRs typically display an opposite enantioselectivity when compared to the well established FMN-dependent Old Yellow Enzymes (OYEs).

中文翻译：

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使用依赖于F ₄₂₀ H _2的酶进行对 映和区域选择性烯还原†

在过去的十年中，很明显，许多微生物都带有使用不常见的黄素辅助因子F _420去氮黄素辅助因子的酶。本文中，我们显示了依赖F _420的还原酶（FDR）可以成功地进行对映体，区域和化学选择性烯的还原。首次，我们证明了F ₄₂₀ H ₂驱动的还原酶可用作生物催化剂，以良好的转化率（> 99％）以及优异的区域选择性和对映体过量（> 99）还原α，β-不饱和酮和醛。 ％ee）。值得注意的是，与公认的FMN依赖的旧黄色酶（OYE）相比，FDR通常显示相反的对映选择性。