AbstractAccurate detection of temperature and oxygen concentration at the cellular level is important in tumor diagnosis. Multifunctional nanocomposites are described that consist of upconversion luminescent nanoparticles capped with mesoporous silica and loaded with an oxygen-sensitive luminescent ruthenium complex. The nanocomposites of type NaYF4:Yb,Er@NaYF4@mSiO2-Ru have two modes of operation: Its red downconversion luminescence (at excitation/emission peaks of 455/606 nm) is quenched by oxygen (O2), and this is used to sense and image O2. The green upconversion luminescence (typically acquired at excitation/emission wavelengths of 980/525 and 544 nm), in turn, is used to measure temperature. The nanocomposites were then applied to dual mode in-vitro imaging of temperature and O2 in hepatocellular carcinoma cells (HepG-2). Graphical abstractA new multifunctional nanocomposite of mesoporous silica coating NaYF4:Yb,Er@NaYF4 upconversion nanopaticless loaded with ruthenium (II) complex has been designed with both green upconversion and red downconversion luminescence for temperature detection, oxygen sensing and dual mode cell imaging.

中文翻译：

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载有钌 (II) 复合纳米粒子的介孔二氧化硅涂层 NaYF4:Yb,Er@NaYF4 上转换纳米粒子：通过上转换和下转换进行氧的荧光传感和细胞成像

摘要 在细胞水平上准确检测温度和氧浓度在肿瘤诊断中具有重要意义。多功能纳米复合材料被描述为由上转换发光纳米颗粒组成，该纳米颗粒覆盖有介孔二氧化硅并负载有氧敏感的发光钌配合物。NaYF4:Yb,Er@NaYF4@mSiO2-Ru 型纳米复合材料有两种工作模式：其红色下转换发光（激发/发射峰为 455/606 nm）被氧气 (O2) 淬灭，这用于感觉和图像 O2。绿色上转换发光（通常在 980/525 和 544 nm 的激发/发射波长下获得）依次用于测量温度。然后将纳米复合材料应用于肝细胞癌细胞 (HepG-2) 中温度和 O2 的双模式体外成像。