The metabolite profile responsible for the quality of mandarin fruit is influenced by preharvest factors including genotype, rootstock, grove location, etc. In this paper, mandarin varieties were discriminated using metabolomics. Additionally, effects on metabolic profiles due to grove location and rootstock differences were also investigated. Results revealed that mandarin varieties could be differentiated using the metabolite profile, while the compositions of flavonoids have the potential for variety differentiation. With regard to fruits of the same variety, grove location might determine the overall profile of metabolites, whereas rootstock possibly affected composition of secondary metabolites. Pathway enrichment analysis demonstrated that biosynthesis pathways of terpenoids and steroids involving limonene and linalool were highly influenced by variety diversity. Moreover, the flavonoid biosynthesis pathway, involving hesperetin, naringenin, eriodictyol, and taxifolin, was indicated to have a close relationship with rootstock differentiation. This study provides useful and important information with depth for breeding and optimizing preharvest practices.

中文翻译：

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普通话（柑桔）的综合代谢组学分析，可作为品种，砧木和树种识别的工具

负责普通话水果品质的代谢产物概况受基因型，砧木，林地位置等收获前因素的影响。在本文中，使用代谢组学来区分普通话品种。另外，还研究了由于树丛位置和砧木差异对代谢谱的影响。结果表明，普通话品种可以通过代谢物谱进行区分，而类黄酮的成分具有进行品种区分的潜力。对于相同品种的水果，树丛的位置可能决定了代谢产物的总体特征，而砧木则可能影响次生代谢产物的组成。途径富集分析表明，涉及柠檬烯和芳樟醇的萜类化合物和类固醇的生物合成途径受到品种多样性的强烈影响。此外，黄酮类化合物的生物合成途径涉及橙皮素，柚皮苷，黄柏醇和滑石粉，表明与砧木分化密切相关。这项研究为繁殖和优化收获前实践提供了有益而重要的信息。