A Novel AuNP‐Based Glucose Oxidase Mimic with Enhanced Activity and Selectivity Constructed by Molecular Imprinting and O2‐Containing Nanoemulsion Embedding,Advanced Materials Interfaces

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A Novel AuNP‐Based Glucose Oxidase Mimic with Enhanced Activity and Selectivity Constructed by Molecular Imprinting and O2‐Containing Nanoemulsion Embedding
Advanced Materials Interfaces ( IF 4.3 ) Pub Date : 2018-09-12 , DOI: 10.1002/admi.201801070
Lin Fan ₁ , Doudou Lou ₁ , Haoan Wu ₁ , Xizhi Zhang ₁ , Yefei Zhu ₂ , Ning Gu ₁ , Yu Zhang ₁

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In spite of the competitive advantages, inorganic nanoparticle mimic enzymes exhibit inherent disadvantages of limited catalytic efficiency and lacking selectivity. Here, a gold nanoparticle (AuNP)‐based mimic enzyme with significantly enhanced glucose selectivity and catalytic activity is constructed and demonstrated for the first time. Aminophenylboronic acid is employed to increase the affinity to glucose, as well as build molecular imprinted polymer shells to realize the selectivity for template molecules of glucose. Besides that, heptadecafluoro‐n‐octyl bromide nanoemulsion with the function of providing oxygen is introduced to gain a further improvement in catalytic activity, which successfully enhances the catalytic efficiency (k_cat/K_m) up to about 270‐fold. Based on the demonstrated catalytic properties, AuNP‐based glucose oxidase mimics are successfully applied in practical glucose detection of drinks and blood glucose.

中文翻译：

一种新型的基于AuNP的葡萄糖氧化酶模拟物，具有分子印迹和含O2纳米乳剂包埋结构，具有增强的活性和选择性。

尽管具有竞争优势，但无机纳米粒子模拟酶仍表现出固有的缺点，即催化效率有限且缺乏选择性。在这里，首次构建并证明了具有显着增强的葡萄糖选择性和催化活性的基于金纳米粒子（AuNP）的模拟酶。氨基苯基硼酸用于增加对葡萄糖的亲和力，并构建分子印迹的聚合物壳以实现对葡萄糖模板分子的选择性。除此之外，还引入了具有提供氧气功能的七氟十六烷-正辛基溴化物纳米乳液，以进一步提高催化活性，从而成功地提高了催化效率（k _cat / K _m）最多约270倍。基于已证明的催化特性，基于AuNP的葡萄糖氧化酶模拟物已成功应用于饮料和血糖的实际葡萄糖检测。

更新日期：2018-09-12

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