Alcohol consumption increases the risk of liver disease. Here, we investigated the effects of Bacillus subtilis co-expressing alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), key enzymes in ethanol degradation, on acute alcohol-induced liver injury. Recombinant B. subtilis significantly alleviated alcohol-induced increase in the liver index, blood alcohol content, and serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities. Furthermore, administration of ADH/ALDH-expressing B. subtilis inhibited lipid peroxidation and oxidative stress in the liver of alcohol-treated mice as evidenced by significant reduction of malondialdehyde and induction of total antioxidant capacity and glutathione and superoxide dismutase levels. Caecal microbiota diversity analysis indicated that administration of recombinant B. subtilis reversed alcohol-induced decrease in Firmicutes and increase in Proteobacteria, in particular pathogenic Helicobacter hepaticus, restoring the microbial composition in alcohol-treated mice. In conclusion, recombinant B. subtilis expressing ADH and ALDH could act as a novel candidate probiotic for prevention of alcohol-induced acute liver injury.

饮酒会增加患肝病的风险。在这里，我们调查了枯草芽孢杆菌共表达酒精脱氢酶（ADH）和乙醛脱氢酶（ALDH）（乙醇降解中的关键酶）对急性酒精引起的肝损伤的影响。重组枯草芽孢杆菌显着减轻了酒精引起的肝脏指数，血液酒精含量以及血清丙氨酸氨基转移酶，天冬氨酸氨基转移酶和碱性磷酸酶活性的增加。此外，施用表达ADH / ALDH的枯草芽孢杆菌丙二醛的显着减少以及总抗氧化剂能力，谷胱甘肽和超氧化物歧化酶水平的诱导证明了抑制酒精治疗小鼠肝脏脂质过氧化和氧化应激的作用。盲肠微生物多样性分析表明重组的施用枯草杆菌逆转酒精诱导降低厚壁菌门和增加变形菌，特别是病原性肝螺杆菌，恢复在醇处理的小鼠的微生物组合物。总之，表达ADH和ALDH的重组枯草芽孢杆菌可以作为预防酒精引起的急性肝损伤的新型候选益生菌。