Ultrathin two-dimensional covalent organic framework nanoprobe for interference-resistant two-photon fluorescence bioimaging†,Chemical Science

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Ultrathin two-dimensional covalent organic framework nanoprobe for interference-resistant two-photon fluorescence bioimaging†
Chemical Science ( IF 7.6 ) Pub Date : 2018-09-10 00:00:00 , DOI: 10.1039/c8sc03393e
Peng Wang ₁ , Fang Zhou ₁ , Cheng Zhang ₁ , Sheng-Yan Yin ₁ , Lili Teng ₁ , Lanlan Chen ₁ , Xiao-Xiao Hu ₁ , Hong-Wen Liu ₁ , Xia Yin ₁ , Xiao-Bing Zhang ₁

Affiliation

The complex environment of living organisms significantly challenges the selectivity of classic small-molecule fluorescent probes for bioimaging. Due to their predesigned topological structure and engineered internal pore surface, covalent organic frameworks (COFs) have the ability to filter out coexisting interference components and help to achieve accurate biosensing. Herein, we propose an effective interference-resistant strategy by creating a COF-based hybrid probe that combines the respective advantages of COFs and small-molecule probes. As a proof of concept, a two-photon fluorescent COF nanoprobe, namely TpASH-NPHS, is developed for targeting hydrogen sulfide (H₂S) as a model analyte. TpASH-NPHS exhibits limited cytotoxicity, excellent photostability and long-term bioimaging capability. More importantly, compared with the small-molecule probe, TpASH-NPHS achieves accurate detection without the interference from intracellular enzymes. This allows us to monitor the levels of endogenous H₂S in a mouse model of cirrhosis.

中文翻译：

用于抗干扰双光子荧光生物成像的超薄二维共价有机骨架纳米探针†

生物体的复杂环境极大地挑战了经典小分子荧光探针用于生物成像的选择性。由于其预先设计的拓扑结构和工程化的内部孔表面，共价有机框架 (COF) 能够过滤掉共存的干扰成分并有助于实现准确的生物传感。在此，我们通过创建一种基于 COF 的混合探针，结合了 COF 和小分子探针的各自优势，提出了一种有效的抗干扰策略。作为概念验证，开发了一种双光子荧光 COF 纳米探针，即TpASH-NPHS，用于靶向硫化氢 (H ₂ S) 作为模型分析物。TpASH-NPHS表现出有限的细胞毒性、优异的光稳定性和长期的生物成像能力。更重要的是，与小分子探针相比，TpASH-NPHS在不受细胞内酶干扰的情况下实现了准确检测。这使我们能够监测肝硬化小鼠模型中内源性 H _{2 S 的水平。}

更新日期：2018-09-10

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