Assembling of hydrophilic and cytocompatible three-dimensional scaffolds based on aminolyzed poly(l-lactide) single crystals†,New Journal of Chemistry

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Assembling of hydrophilic and cytocompatible three-dimensional scaffolds based on aminolyzed poly(l-lactide) single crystals†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-09-10 00:00:00 , DOI: 10.1039/c8nj03205j
Ahmed Bakry _{1,

2,

3,

4,

5} , Mohamed S. A. Darwish _{5,

6,

7} , Ahmed M. A. El Naggar _{5,

6,

7}

Affiliation

Poly(L-lactide) exhibits good biocompatibility and processability, but its surface lacks proper hydrophilicity for cell growth and proliferation, which hinders its use as a tissue engineering scaffold. Surface aminolysis has been reported to improve the surface hydrophilicity and cytocompatibility of polymers. However, the aminolysis of 2D substrates has been achieved on the surface to only low depths, and aminolysis of 3D scaffolds does not guarantee homogenous distribution of amine functionality on their surfaces. Thus, herein, we address these problems by developing 3D scaffolds based on pre-aminolyzed poly(L-lactide) single crystals. The scaffolds are fabricated using the simple compression molding salt leaching technique in the absence of heat and gluing materials. Mechanical and morphological studies show that the scaffolds are mechanically stable with well interconnected open-porous structures. Furthermore, the ninhydrin test confirms homogenous spatial distribution of amine groups. The biological behavior of the scaffolds is investigated by seeding them with fibroblast cells. The aminolyzed single crystals offer a better interface for fibroblasts to adhere, proliferate and migrate than the pristine crystals and hence result in a promising 3D porous structure for tissue engineering applications.

中文翻译：

基于氨基分解的聚（l-丙交酯）单晶的亲水性和细胞相容性三维支架的组装†

聚（L-丙交酯）具有良好的生物相容性和可加工性，但其表面缺乏适合细胞生长和增殖的亲水性，这阻碍了其用作组织工程支架。据报道，表面氨解可改善聚合物的表面亲水性和细胞相容性。然而，2D底物的氨解仅在表面上实现了很低的深度，而3D支架的氨解不能保证胺官能团在其表面上的均匀分布。因此，在本文中，我们通过开发基于预氨基化的聚（L-丙交酯）单晶。在没有热和胶合材料的情况下，使用简单的压模盐浸技术制造支架。力学和形态学研究表明，所述支架在机械上是稳定的，具有良好互连的开孔结构。此外，茚三酮测试证实了胺基团的均匀空间分布。通过将支架植入成纤维细胞来研究支架的生物学行为。相比于原始晶体，经过氨基水解的单晶为成纤维细胞提供了更好的界面粘附，增殖和迁移，因此可为组织工程应用提供前景广阔的3D多孔结构。

更新日期：2018-09-10

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