Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-08-11 , DOI: 10.1007/s00216-018-1303-4 Yudai Tabuchi , Masumi Taki
We established a novel principle for fluorescence detection of a target protein. A low-molecular-weight fluorescent pharmacophore, as a targeted probe, was selected from a dynamic combinatorial library of Schiff bases. The pharmacophore retains its fluorescence when bound to the hydrophobic site of the target, whereas it loses it because of hydrolysis when unbound.
We describe a novel concept for detection of a target protein (i.e., HSA) by using a keep-on-type fluorescent pharmacophore which is discovered from a dynamic combinatorial library of Schiff bases. When the target is absent, the keep-on-pharmacophore is degraded by hydrolysis, with the result that we can see no fluorescence.
中文翻译：
从席夫碱的动态组合库获得的荧光“保持”型药效团
我们建立了一种新的原理，用于目标蛋白的荧光检测。从Schiff碱基的动态组合库中选择一种低分子量荧光药效基团作为目标探针。当与目标的疏水位点结合时，药效基团保留其荧光，而当未结合时，其由于水解而丢失。
我们描述了一种新的概念，用于通过使用从Schiff碱基的动态组合库中发现的保持型荧光药效基团来检测目标蛋白（即HSA）。当不存在靶标时，保持药效基团被水解降解，结果我们看不到荧光。
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