Abstract Photochemical derivatization (at 253 nm) of varenicline in alkaline medium (in NaOH 0.64 mol L−1) was successfully achieved aiming to induce strong fluorescence for quantitative purposes. The intense fluorescence from the produced photo-derivative covered a linear range of three orders of magnitude (10−8–10−6 mol L−1 range) allowing the spectrofluorimetric determination of the analyte in pharmaceutical formulations. For urine, the UV exposed sample was analyzed by high-performance liquid chromatography using borate buffer (0.01 mol L−1, pH 10.0). Separations were made using a C18 column with borate buffer (0.01 mol L−1, pH 10)/methanol (30:70% v/v) in isocratic elution. Detection limit was 2.5 × 10−7 mol L−1 for determination using HPLC. Measurement uncertainty was estimated and both methods were applied for the determination of varenicline in a pharmaceutical preparation and urine samples with acceptable recoveries using no expensive and toxic derivatization dyes.

中文翻译：

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使用荧光分光光度法和高效液相色谱法测定光化学荧光增强后的伐尼克兰

摘要 伐尼克兰在碱性介质（NaOH 0.64 mol L-1）中的光化学衍生（253 nm）已成功实现，旨在诱导强荧光用于定量目的。来自产生的光衍生物的强荧光覆盖了三个数量级的线性范围（10-8-10-6 mol L-1 范围），允许对药物制剂中的分析物进行分光荧光测定。对于尿液，使用硼酸盐缓冲液（0.01 mol L-1，pH 10.0）通过高效液相色谱分析暴露于紫外线的样品。使用 C18 柱和硼酸盐缓冲液（0.01 mol L-1，pH 10）/甲醇（30:70% v/v）等度洗脱进行分离。使用 HPLC 测定的检测限为 2.5 × 10-7 mol L-1。