Laccases are used in decolorization and biodegradation of synthetic dyes, bioremediation of industrial wastewaters and delignification of lignocellulosic compounds. The aims of the present study were the optimization of a recombinant laccase production in Yarrowia lipolytica yeast using sucrose as a main carbon source, and the application of the resulting enzyme to decolorization of synthetic dyes, which are problematic environmental pollutants. Taguchi's experimental design method was employed to optimize medium compounds. Recombinant laccase production by Y. lipolytica YL4 strain increased to 900 U L⁻¹ after optimization of sucrose, ammonium chloride, yeast extract and thiamine levels in the modified PPB medium. Furthermore, the production rate reached 6760 U L⁻¹ in a 5 L bioreactor which represents 4.5- and 33.5-fold increases compared to cultures that were in shake-flask with optimized and primary media, respectively. The supernatant containing secreted recombinant laccase was applied for decolorization of seven dyes. The effects of pH, the amount of enzyme and incubation period were verified. The effect of incubation time on dye decolorization by recombinant laccase was important, which has an influence of greater extent than 90% after 48 h for all dyes. The Trametes versicolor laccase can be efficiently produced in Y. lipolytica and the recombinant enzyme has a considerable potential in the decolorization of pollutant synthetic dyes.

漆酶用于合成染料的脱色和生物降解，工业废水的生物修复和木质纤维素化合物的脱木质素。本研究的目的是利用蔗糖作为主要碳源，优化解脂耶氏酵母酵母中重组漆酶的生产，并将所得酶用于合成染料的脱色，合成染料是有问题的环境污染物。Taguchi的实验设计方法用于优化培养基化合物。解脂耶氏酵母YL4菌株产生的重组漆酶增加至900 UL ^-1在改良的PPB培养基中优化蔗糖，氯化铵，酵母提取物和硫胺素水平后进行。此外，在5 L生物反应器中，生产率达到6760 UL ^-1，与分别在摇瓶中使用优化培养基和原始培养基的培养物相比，生产率提高了4.5倍和33.5倍。将含有分泌的重组漆酶的上清液用于七种染料的脱色。验证了pH，酶量和孵育时间的影响。孵育时间对重组漆酶对染料脱色的影响很重要，对所有染料而言，在48小时后，其影响程度均大于90％。的变色栓菌的漆酶可以高效地生产解脂亚罗威阿酵母 重组酶在污染物合成染料的脱色方面具有相当大的潜力。