This proposed work aimed to investigate the chemical characteristic, antioxidant capacities and hepatoprotection effect of pomegranate peel polysaccharides (PPP) on CCl₄-induced oxidative damage in mice. PPP was identified as the acidic heteropolysaccharides by HPLC methods. In vitro test showed that PPP had excellent reducing power and scavenging effects against free radicals. Administration of PPP (50, 100 and 200 mg/kg·bw) in mice before the injection of CCl₄ could observably antagonize the increased serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and hepatic malonaldehyde level in CCl₄-induced mice, especially administrated with 200 mg/kg·bw of PPP. Hepatic enzymatic activities of total superoxide dismutase, glutathione peroxidase, catalase and non-enzymatic activity of glutathione were markedly increased at high dosage of PPP, respectively. In addition, histopathological observation of liver further proved these biochemical characteristics. Therefore, it can be concluded that PPP exhibits strong protective effects against CCl₄-induced liver injury in mice.

这项拟议的工作旨在研究石榴皮多糖（PPP）对CCl ₄诱导的小鼠氧化损伤的化学特性，抗氧化能力和肝保护作用。通过HPLC方法将PPP鉴定为酸性杂多糖。体外试验表明，PPP具有优异的还原能力和清除自由基的作用。PPP的给药（50，100和200毫克/千克·体重）在小鼠中注射四氯化碳的前₄可以观察地拮抗四氯化碳增加的血清谷丙转氨酶，天冬氨酸转氨酶，碱性磷酸酶和肝丙二醛水平₄诱导的小鼠，尤其是给予200 mg / kg·bw的PPP。在高剂量PPP下，总超氧化物歧化酶，谷胱甘肽过氧化物酶，过氧化氢酶的肝酶活性和谷胱甘肽的非酶活性分别显着增加。另外，肝脏的组织病理学观察进一步证明了这些生化特征。因此，可以得出结论，PPP对小鼠的CCl ₄诱导的肝损伤具有很强的保护作用。