Glycoconjugates extracted from Genipa americana leaves (PE-Ga) were separated into two fractions, denominated as PFI and PFII (total carbohydrate: 23-36%/uronic acid: 9-30%; protein:4-5%; polyphenols:0.776-0.812 mg/g), mainly composed by arabinose, galactose and uronic acid and presenting high (PFI) and low (PFII) molecular weight (based on polyacrylamide electrophoresis gel and gel permeation chromatography). Uronic acid was also detected by FT-IR (wavenumbers: 1410 and 1333 cm^-1) and NMR (α-GalpA). Deproteinization of glycoconjugates showed reduced protein and polyphenol levels with loss of its biological effects. PE-Ga and PFII prolonged clotting time-aPTT (3.6 and 1.8x), while PE-Ga and PFI inhibited by 48% (100 µg/µL) the ADP-induced platelet aggregation. In vivo, these glycoconjugates at 1 mg/kg inhibited (37-53%) venous thrombus formation (4.7 ± 0.1 mg) and increased bleeding time (PE-Ga and PFI:3.0x; PFII:1.7x vs. PBS:906 ± 16.7 s). In conclusion, the arabinogalactan-rich glycoconjugate of G. americana leaves, containing uronic acid, present antiplatelet, anticoagulant (intrinsic/common pathway) and antithrombotic effects, with low hemorrhagic risk.

从美洲番石榴叶（PE-Ga）中提取的糖结合物被分为两部分，分别命名为PFI和PFII（总碳水化合物：23-36％/糖醛酸：9-30％;蛋白质：4-5％;多酚：0.776- 0.812 mg / g），主要由阿拉伯糖，半乳糖和糖醛酸组成，具有高（PFI）和低（PFII）分子量（基于聚丙烯酰胺电泳凝胶和凝胶渗透色谱法）。：糖醛酸也通过FT-IR（1410和1333厘米波数检测^-1和NMR（α-Gal的）p一个）。糖缀合物的去蛋白显示蛋白质和多酚水平降低，同时丧失其生物学作用。PE-Ga和PFII延长了凝血时间aPTT（3.6和1.8倍），而PE-Ga和PFI则抑制了ADP诱导的血小板凝集48％（100 µg / µL）。在体内，这些糖缀合物以1 mg / kg的剂量抑制（37-53％）静脉血栓形成（4.7±0.1 mg），并增加了出血时间（PE-Ga和PFI：3.0x； PFII：1.7x vs. PBS：906± 16.7秒）。总之，含有糖醛酸的美国G.叶富含阿拉伯半乳聚糖的糖结合物具有抗血小板，抗凝血（内在/共同途径）和抗血栓形成的作用，出血风险低。