An efficient analytical method based on molecularly imprinted solid-phase extraction (MISPE) coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) was established for the determination of iridoid glycosides (IGs) in Di-huang pills. As the solid-phase extraction medium, superhydrophilic molecularly imprinted polymers (MIPs) with high affinity and selectivity to IGs in water media were fabricated using divinyl galactose as a single cross-linking monomer. The structure, porosity, and hydrophilicity of MIPs were characterized. The properties involving dynamic adsorption, kinetic adsorption, and selectivity were evaluated. Under optimal conditions the MISPE-HPLC-DAD based method was applied for loganin, morroniside, cornin, and sweroside determination in three kinds of Di-huang pills. The limits of detection of four IGs were 0.002–0.003 mg g⁻¹. Furthermore, the proposed method exhibited some merits including good linearity, excellent precision, and desirable accuracy. The established MISPE-HPLC-DAD method has great potential for the selective determination of IGs in Chinese patent drugs.

Open image in new window

建立了在环烯醚萜甙（IGS）的确定基于加上高效液相色谱法二极管阵列检测（HPLC-DAD）分子印迹固相萃取（MISPE）一种有效的分析方法迪-黄药。作为固相萃取介质，使用二乙烯基半乳糖作为单一交联单体，制备了对水介质中IG具有高亲和力和选择性的超亲水分子印迹聚合物（MIP）。表征了MIP的结构，孔隙率和亲水性。评价了涉及动态吸附，动态吸附和选择性的性质。在最佳条件下在3种应用于马钱苷，莫诺，cornin和sweroside确定MISPE-HPLC-DAD为基础的方法迪-黄丸。四个IG的检出限为0.002-0.003 mg g ^-1。此外，所提出的方法表现出一些优点，包括良好的线性，优异的精度和期望的精度。所建立的MISPE-HPLC-DAD方法具有选择性测定中成药中IGs的巨大潜力。

在新窗口中打开图像