We investigated the protective effects and possible mechanisms of Aplysin against alcohol-induced liver injury. Rats were given daily either alcohol only (alcohol model group; 8 to 12 mL/kg body weight), one of three doses of Aplysin (50, 100, or 150 mg/kg Aplysin) plus alcohol, or volume-matched saline. After 6 weeks, the effects of Aplysin were assessed in terms of changes in histology, biochemical indices, and DNA oxidative damage. Potential mechanisms were analyzed through measurements of lipid peroxidation, antioxidant defense systems, expression of cytochrome P450 2E1, and expression of apoptosis-related genes. We found that Aplysin significantly protected the liver against alcohol-induced oxidative injury, evidenced by improved hepatic histological structure, inhibited alcohol-induced elevation of serum biochemical indices, attenuated extents of hepatocellular DNA damage. At a mechanistic level, Aplysin alleviated alcohol-induced oxidative stress as illustrated by the revivification of erythrocyte membrane fluidity, the attenuation of glutathione depletion, the restoration of antioxidase activities, and reduced malondialdehyde overproduction. Furthermore, the mRNA levels of Bax, cytochrome c, and cytochrome P450 2E1 were significantly down-regulated, whereas those of Bcl-2 and caspase-9 and caspase-3 were markedly up-regulated. These findings suggest that Aplysin provides significant protection against alcohol-induced liver injury, possibly through alleviating oxidative damage and modulating endogenous apoptosis-related genes expression. PRACTICAL APPLICATION Many natural components derived from alga have been used in the food, cosmetics, and biomedicine industries. Aplysin, a marine bromosesquiterpene, was extracted from the red alga Laurencia tristicha, which could effectively protect against alcohol-induced liver injury, might be a potential natural sources for preventing alcoholic liver damage.

中文翻译：

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Aplysin 通过减轻氧化损伤和调节大鼠内源性凋亡相关基因的表达来防止酒精引起的肝损伤

我们研究了海兔毒素对酒精性肝损伤的保护作用和可能的机制。每天只给大鼠服用酒精（酒精模型组；8 至 12 mL/kg 体重）、三种剂量的 Aplysin（50、100 或 150 mg/kg Aplysin）中的一种加酒精，或体积匹配的盐水。6 周后，根据组织学、生化指标和 DNA 氧化损伤的变化评估海兔肽的作用。通过测量脂质过氧化、抗氧化防御系统、细胞色素 P450 2E1 的表达和凋亡相关基因的表达来分析潜在的机制。我们发现海兔素显着保护肝脏免受酒精引起的氧化损伤，这可以通过改善肝脏组织学结构、抑制酒精引起的血清生化指标升高来证明，减轻肝细胞 DNA 损伤的程度。在机制水平上，海兔肽缓解了酒精诱导的氧化应激，这可以通过红细胞膜流动性的恢复、谷胱甘肽消耗的减弱、抗氧化酶活性的恢复和丙二醛过量产生的减少来说明。此外，Bax、细胞色素 c 和细胞色素 P450 2E1 的 mRNA 水平显着下调，而 Bcl-2 和 caspase-9 和 caspase-3 的 mRNA 水平显着上调。这些发现表明，海兔肽对酒精引起的肝损伤提供了显着的保护作用，可能是通过减轻氧化损伤和调节内源性细胞凋亡相关基因的表达。实际应用 许多源自藻类的天然成分已用于食品、化妆品和生物医药行业。