Gold nanoparticles and reduced graphene oxide-amplified label-free DNA biosensor for dasatinib detection,New Journal of Chemistry

当前位置： X-MOL 学术 › New J. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Gold nanoparticles and reduced graphene oxide-amplified label-free DNA biosensor for dasatinib detection
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2018-09-06 , DOI: 10.1039/c8nj03783c
Fahimeh Tahernejad-Javazmi _{1,

2,

3,

4,

5} , Mehdi Shabani-Nooshabadi _{1,

2,

3,

4,

5} , Hassan Karimi-Maleh _{5,

6,

7,

8,

9}

Affiliation

Dasatinib or sprycel is an anticancer drug for treatment of chronic myelogenous leukemia, prostate cancer, and some of the other cancers with several adverse effects. In this research, we suggest an amplified biosensor, based on a glassy carbon electrode modified with ds-DNA, for detection of dasatinib in aqueous solutions. The study also recommends reduced graphene oxide (rGO) and gold nanoparticles (Au-NPs) (Au-NPs/rGO/ds-DNA/GC) for the same purpose. The Au-NPs/rGO/GC was/were characterized using Atomic Force Microscopy (AFM) and Electrochemical Impedance Spectroscopy (EIS) methods. The guanine oxidation peak current of ds-DNA was selected as a signal for the analysis of dasatinib in the concentration range of 0.03–5.5 μM. This was carried out with the help of the differential pulse voltammetry method. Moreover, binding energy investigation showed an equilibrium constant (K_a) equal to 2.25 × 10⁵ M⁻¹ between dasatinib and ds-DNA. The Au-NPs/rGO/ds-DNA/GC was used for dasatinib analysis in tablet and urine samples.

中文翻译：

金纳米颗粒和还原型氧化石墨烯扩增的无标记DNA生物传感器用于达沙替尼检测

达沙替尼或sprycel是一种抗癌药，用于治疗慢性粒细胞性白血病，前列腺癌和其他一些具有多种不良反应的癌症。在这项研究中，我们建议基于ds-DNA修饰的玻碳电极的扩增生物传感器，用于检测水溶液中的达沙替尼。该研究还建议出于相同目的，还原氧化石墨烯（rGO）和金纳米颗粒（Au-NPs）（Au-NPs / rGO / ds-DNA / GC）。使用原子力显微镜（AFM）和电化学阻抗谱（EIS）方法对Au-NPs / rGO / GC进行了表征。选择ds-DNA的鸟嘌呤氧化峰电流作为0.03–5.5μM浓度范围内达沙替尼分析的信号。这是借助于差分脉冲伏安法进行的。而且，达沙替尼和ds-DNA之间的K _a）等于2.25×10 ⁵ M ^-1。Au-NPs / rGO / ds-DNA / GC用于片剂和尿液样品中的达沙替尼分析。

更新日期：2018-09-25

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11