Encapsulation of laccase within zwitterionic poly-carboxybetaine hydrogels for improved activity and stability,Catalysis Science & Technology

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Encapsulation of laccase within zwitterionic poly-carboxybetaine hydrogels for improved activity and stability
Catalysis Science & Technology ( IF 4.4 ) Pub Date : 2018-09-06 00:00:00 , DOI: 10.1039/c8cy01460d
Yingnan Zhu _{1,

2,

3,

4,

5} , Jiayin Song _{1,

2,

3,

4,

5} , Jiamin Zhang _{1,

2,

3,

4,

5} , Jing Yang _{1,

2,

3,

4,

5} , Weiqiang Zhao _{1,

2,

3,

4,

5} , Hongshuang Guo _{1,

2,

3,

4,

5} , Tong Xu _{1,

2,

3,

4,

5} , Xiao Zhou _{3,

5,

6,

7,

8} , Lei Zhang _{1,

2,

3,

4,

5}

Affiliation

Laccase is an important oxidase for many applications such as bioremediation, chemical synthesis, and biosensors, due to its wide range of substrates and prominent capacity to degrade phenolic compounds. However, its unsatisfactory stability, activity, and reusability still remain a major challenge for practical applications. In this work, we found that when laccase was encapsulated in zwitterionic hydrogels, especially poly-carboxybetaine (PCB), its performance could be significantly enhanced. Compared with the free enzyme, PCB–laccase displayed a remarkably increased activity (∼20-fold higher K_cat/K_m value). Meanwhile, its stability could also be effectively maintained even in an extensive range of temperatures, pH values and organic solvents. In addition, PCB–laccase demonstrated excellent storage stability, and retained 80% of its initial activity after an 8-week storage period. In a successive 6-cycle decoloration of acid orange 7, PCB–laccase could still retain 52% of its initial activity, demonstrating superior durability to the free laccase. Therefore, we have provided an efficient and stable support matrix for enzyme immobilization, which exhibits potential for practical applications even in harsh environments.

中文翻译：

两性离子聚羧基甜菜碱水凝胶中漆酶的包裹，以提高活性和稳定性

漆酶是多种应用（例如生物修复，化学合成和生物传感器）中的重要氧化酶，因为它具有广泛的底物范围和显着的降解酚类化合物的能力。但是，其令人满意的稳定性，活性和可重复使用性仍然是实际应用中的主要挑战。在这项工作中，我们发现将漆酶封装在两性离子水凝胶中，尤其是聚羧基甜菜碱（PCB）中时，其性能可以得到显着提高。与游离酶相比，PCB-漆酶的活性显着增加（K _cat / K _m约高20倍）价值）。同时，即使在广泛的温度，pH值和有机溶剂范围内，也可以有效地保持其稳定性。另外，PCB-漆酶具有出色的储存稳定性，并且在储存8周后仍保留了其初始活性的80％。在连续6个周期的酸性橙7脱色过程中，PCB漆酶仍可保留其初始活性的52％，显示出优于游离漆酶的耐久性。因此，我们提供了一种高效稳定的酶固定载体基质，即使在恶劣的环境中也具有实际应用的潜力。

更新日期：2018-09-06

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