AbstractThe adhesion force of the tentacle of a live cypris on a glass surface covered with polymer brush was directly measured by scanning probe microscopy. Polymer brushes were prepared on the cover glass and silicon wafers by surface-initiated atom transfer radical polymerization of 3-(N-2-methacryloyloxyethyl-N,N-dimethyl)ammonatopropanesulfonate (MAPS) or 2-hydroxyethyl methacrylate (HEMA). A small amount of glue was placed at the edge of a tipless cantilever supported by the piezo motion control system of the scanning probe microscope and monitored by using an optical microscope. A live cypris swimming in seawater was held down on the slide glass by the cantilever for 30 min until the glue cured. The tentacles of the live cypris immobilized on the cantilever were forced to make contact with the surface-modified cover glass. The adhesion force was determined by the torsion of the cantilever when the tentacle was detached from the cover glass surface. The adhesion force between the cypris and the propylsilane-modified glass surface increased from 1.6 to 40 μN with the increase in age of the cypris larva from 3 to 21 days after metamorphosis from the nauplius larva. Poly(MAPS) and poly(HEMA) brush surfaces exhibited extremely low adhesion to the cypris larva during 21 days in seawater, indicating the effective antifouling property of hydrophilic polymer brushes.The adhesion force between the tentacle of a live cypris and polymer brushes on the sidewall of cover glass was directly measured by scanning probe microscopy in seawater. The cypris was immobilized on the cantilever with glue, and then forced to make contact with the modified surface. When the tentacle was detached by moving the cantilever away from the surface, the torsion of the cantilever caused the laser deflection on the photodiode corresponding to the adhesion force. Polymer brush surfaces exhibited extremely low adhesion to the cypris larva.

中文翻译：

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用扫描探针显微镜测量海水中活鲤鱼触手的附着力

摘要 利用扫描探针显微镜直接测量了活鲤触手在聚合物刷覆盖的玻璃表面上的粘附力。通过表面引发的原子转移自由基聚合 3-(N-2-甲基丙烯酰氧基乙基-N,N-二甲基) 氨基丙磺酸酯 (MAPS) 或 2-羟乙基甲基丙烯酸酯 (HEMA) 在盖玻片和硅片上制备聚合物刷。在扫描探针显微镜压电运动控制系统支撑的无尖悬臂边缘放置少量胶水，并使用光学显微镜进行监测。一只在海水中游泳的活鲤鱼被悬臂固定在载玻片上 30 分钟，直到胶水固化。固定在悬臂上的活 cypris 的触手被迫与表面改性的盖玻片接触。当触手从盖玻片表面分离时，粘附力由悬臂的扭转决定。从无节幼体幼虫变态后的3天到21天，随着鲤鱼幼虫年龄的增加，鲤鱼与丙基硅烷改性玻璃表面之间的粘附力从1.6增加到40μN。Poly(MAPS)和poly(HEMA)刷表面在海水中21天后对鲤鱼幼虫的附着力极低，表明亲水聚合物刷具有有效的防污性能。通过扫描探针显微镜在海水中直接测量盖玻片的侧壁。cypris 用胶水固定在悬臂上，然后被迫与改性表面接触。当通过将悬臂从表面移开而使触手脱离时，悬臂的扭转导致光电二极管上的激光偏转与粘附力相对应。聚合物刷表面对鲤鱼幼虫的附着力极低。