This study aims to reveal the effects of pressure during cold isostatic pressing (CIP) on the microstructure and optical properties of fluorescent HAp complexes. Although the microsturucture-dependent properties of fluorescent HAp complexes have been reported to improve the antibacterial properties of photocatalyst coating layers, the mechanism behind the changes in the fluorescence properties of highly compressed HAp complexes has not yet been unveiled. CIP was successfully used to fabricate fluorescent HAp – amino acid complexes, and their fluorescence intensities increased with increasing fabrication pressure. Peak wavelength of fluorescence emitted by the HAp – amino acid complexes exhibited yellow to red shift. Although the thickness of the amino acid layer was saturated in higher pressure cases, the concentration of amino acids increased proportionally with pressure, which suggests changes in the packing structures of the ligands in the HAp– amino acid complexes. Polarized Raman spectroscopy measurements clearly detected ligands normally arranged to the HAp layer under high pressure fabrication conditions, which can provide the tightly packed ligand structure in the HAp– amino acid complexes. These tightly packed ligand structure in the HAp– amino acid complexes could emit stronger fluorescence owing to the increased density of complexations. This newly found pressure dependency in the optical properties of HAp–amino acid complexes is beneficial for developing biocompatible fluorescence materials or enhancement agents for antibacterial coating layers.

这项研究旨在揭示冷等静压（CIP）期间压力对荧光HAp配合物的微观结构和光学性质的影响。尽管已经报导了荧光HAp配合物的微结构依赖性，可以改善光催化剂涂层的抗菌性能，但尚未揭示高度压缩的HAp配合物的荧光性质改变的机理。CIP已成功用于制造荧光HAp-氨基酸复合物，并且其荧光强度随制造压力的增加而增加。HAp –氨基酸复合物发出的荧光的峰值波长显示出黄变红。虽然在高压情况下氨基酸层的厚度饱和，氨基酸的浓度随压力成比例增加，这表明HAp-氨基酸复合物中配体的堆积结构发生了变化。极化拉曼光谱法测量可以清楚地检测到在高压制造条件下通常排列在HAp层上的配体，可以在HAp-氨基酸复合物中提供紧密堆积的配体结构。由于复合物密度的增加，HAp-氨基酸复合物中这些紧密堆积的配体结构可能发出更强的荧光。这种新发现的对HAp-氨基酸复合物光学特性的压力依赖性，有利于开发生物相容性荧光材料或抗菌涂层的增强剂。这表明HAp-氨基酸复合物中配体的堆积结构发生了变化。极化拉曼光谱法测量可以清楚地检测到在高压制造条件下通常排列在HAp层上的配体，可以在HAp-氨基酸复合物中提供紧密堆积的配体结构。由于复合物密度的增加，HAp-氨基酸复合物中这些紧密堆积的配体结构可能发出更强的荧光。这种新发现的对HAp-氨基酸复合物光学特性的压力依赖性，有利于开发生物相容性荧光材料或抗菌涂层的增强剂。这表明HAp-氨基酸复合物中配体的堆积结构发生了变化。极化拉曼光谱法测量可以清楚地检测到在高压制造条件下通常排列在HAp层上的配体，这可以在HAp-氨基酸复合物中提供紧密堆积的配体结构。由于复合物密度的增加，HAp-氨基酸复合物中这些紧密堆积的配体结构可能发出更强的荧光。这种新发现的对HAp-氨基酸复合物光学特性的压力依赖性有利于开发生物相容性荧光材料或用于抗菌涂层的增强剂。极化拉曼光谱法测量可以清楚地检测到在高压制造条件下通常排列在HAp层上的配体，这可以在HAp-氨基酸复合物中提供紧密堆积的配体结构。由于复合物密度的增加，HAp-氨基酸复合物中这些紧密堆积的配体结构可能发出更强的荧光。这种新发现的对HAp-氨基酸复合物光学特性的压力依赖性，有利于开发生物相容性荧光材料或抗菌涂层的增强剂。极化拉曼光谱法测量可以清楚地检测到在高压制造条件下通常排列在HAp层上的配体，这可以在HAp-氨基酸复合物中提供紧密堆积的配体结构。由于复合物密度的增加，HAp-氨基酸复合物中这些紧密堆积的配体结构可能发出更强的荧光。这种新发现的对HAp-氨基酸复合物光学特性的压力依赖性，有利于开发生物相容性荧光材料或抗菌涂层的增强剂。