Protein functionalized micro-scale patterned structures are developed using a biocompatible polymer PLGA (poly (d, l-lactide-co-glycolide)) via thin film dewetting and by step-wise chemical conjugations with EDA (ethylenediamine) and anti-EpCAM (Epithelial Cell Adhesion Molecule) antibodies to target the epithelial cell adhesion molecules of cancer cells. The effectiveness of such protein functionalized patterned surface is checked through cell isolation process using blood samples spiked with different cancer cells such as MCF-7, A549, MDA-MB-231. An efficient capture yield of 92% is obtained with MCF-7 cells over a two hour incubation time. The study demonstrates the effects of cell concentration and incubation time on the binding of cancer cells to the modified patterned surfaces. For the first time, a simple and inexpensive method is reported to fabricate functionalized PLGA patterned surface for an efficient isolation of cancer cells from diluted blood samples. The method shows the potential to be used as an effective platform for the development of an improved circulating tumor cell (CTC) isolation device from the clinical blood sample.

使用生物相容性聚合物PLGA（poly（d，l-丙交酯-乙交酯乙交酯）通过薄膜去湿以及与EDA（乙二胺）和抗EpCAM（上皮细胞粘附分子）抗体的逐步化学结合来靶向癌细胞的上皮细胞粘附分子。通过使用分离了不同癌细胞（例如MCF-7，A549，MDA-MB-231）的血液样本的细胞分离过程，检查了这种蛋白质功能化的图案化表面的有效性。在两小时的培养时间内，使用MCF-7细胞可获得92％的有效捕获率。该研究证明了细胞浓度和孵育时间对癌细胞与修饰的图案化表面结合的影响。首次，据报道，一种简单且廉价的方法可制造功能化的PLGA图案化表面，以从稀释的血液样本中有效分离癌细胞。该方法显示出有潜力用作从临床血液样本开发改进的循环肿瘤细胞（CTC）分离装置的有效平台。