Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells,Bioconjugate Chemistry

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Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2018-09-05 00:00:00 , DOI: 10.1021/acs.bioconjchem.8b00558
Ala Jo , June Sung , Sanghee Lee ₁ , Hyunsung Nam , Hyo Won Lee , Jongmin Park ₂ , Hwan Myung Kim , Eunha Kim , Seung Bum Park

Affiliation

Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO₂H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO₂H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.

中文翻译：

近红外荧光示踪剂，用于监测活细胞中的葡萄糖摄取

通过表型筛选系统的开发，用于葡萄糖摄取监测的荧光示踪剂可以用作诊断和发现新型治疗剂的化学工具。在这里，我们提出了一种新的近红外荧光葡萄糖示踪剂Glc-SiR-CO ₂ H，用于监测细胞对葡萄糖的摄取。通过将葡糖胺与两种不同的若丹明硅荧光染料共轭，我们发现荧光染料的净电荷对探针的细胞摄取具有相当大的影响。用d / l-葡萄糖进行的竞争测定以及蛋白质印迹分析暗示了该探针的GLUT依赖性摄取机制。最后，Glc-SiR-CO ₂H不仅可以将癌细胞与正常细胞区分开，还可以监测活细胞中的抗癌作用。

更新日期：2018-09-05

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