Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice†,Chemical Science

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Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice†
Chemical Science ( IF 7.6 ) Pub Date : 2018-09-05 00:00:00 , DOI: 10.1039/c8sc03226b
Xinfu Zhang _{1,

2} , Weiyu Zhao ₁ , Bin Li ₁ , Wenqing Li ₁ , Chengxiang Zhang ₁ , Xucheng Hou ₁ , Justin Jiang ₁ , Yizhou Dong _{1,

3,

4,

5,

6,

7}

Affiliation

Hypochlorous acid (HClO) is a promising diagnostic marker for inflammation and relevant diseases. Although many probes were previously developed for HClO imaging, the development of organ targeting probes is still lacking. Herein, we designed and synthesized a series of cyanine derivatives as ratiometric fluorescent probes to detect endogenous HClO in the lungs with inflammation. By installing diverse lipid chains and amino groups on cyanine, we identified that ClO1, with one n-octadecane chain and two 2-[[2-(dimethylamino)ethyl]methylamino]-ethyl groups, is a superior probe to target the lungs over other major organs in mice. ClO1 was able to sense both exogenous and endogenous HClO in A549 (human lung epithelial) cells through fluorescence ratiometric imaging. In a lipopolysaccharide (LPS)-induced lung inflammation mouse model, ClO1 effectively captured endogenous HClO in the lungs after intravenous administration. Overall, these cyanine-derived probes merit further development as organ targeting HClO sensors.

中文翻译：

用于捕获小鼠肺部内源性次氯酸的比率荧光探针†

次氯酸 (HClO) 是一种有前途的炎症和相关疾病的诊断标记物。尽管之前开发了许多用于 HClO 成像的探针，但器官靶向探针的开发仍然缺乏。在此，我们设计并合成了一系列花青衍生物作为比例荧光探针，用于检测炎症肺部的内源性HClO。通过在花青上安装不同的脂质链和氨基，我们发现ClO1具有一个正十八烷链和两个 2-[[2-(二甲基氨基)乙基]甲基氨基]-乙基，是一种针对肺部的优异探针。小鼠的其他主要器官。ClO1能够通过荧光比例成像检测 A549（人肺上皮）细胞中的外源性和内源性 HClO。在脂多糖 (LPS) 诱导的肺部炎症小鼠模型中，ClO1静脉注射后可有效捕获肺部内源性 HClO。总体而言，这些花青衍生的探针值得进一步开发作为针对 HClO 传感器的器官。

更新日期：2018-09-05

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