Kenaf seed oil-in-water nanoemulsions (NANO) stabilized by sodium caseinate (SC), beta-cyclodextrin (β-CD), and Tween 20 (T20) have been optimized and shown to improve in vitro bioaccessibility and physicochemical stability in the previous study. The main objective of this study was to evaluate the stability of bioactive compounds and antioxidants in the NANO during storage at different temperatures (4 °C, 25 °C, and 40 °C). An evaluation of the antioxidant activities of each emulsifier showed that SC had good scavenging capability with 97.6% ABTS radical scavenging activity. Therefore, SC which was used as one of the main emulsifiers could further enhanced the antioxidant activity of NANO. At week 8 of storage, NANO that stored at 4 °C had maintained the best bioactive compounds stability and antioxidant activities with 90% retention of vitamin E and 65% retention of phytosterols. These results suggested that 4 °C would be the most suitable storage temperature for NANO containing naturally present vitamin E and phytosterols. From the accelerated storage results at 40 °C, NANO containing vitamin E and phytosterols had maintained half of its initial concentration until week 4 and week 2 of storage, which is equivalent to 16 weeks and 8 weeks of storage at room temperature, respectively. PRACTICAL APPLICATION The results of this study provide a better understanding on the stability of bioactive compounds and antioxidant activities in oil-in-water nanoemulsions that stabilized by similar ternary emulsifiers during storage at different temperatures. In addition, this study could be used as a predictive model to estimate the shelf life of bioactive compounds encapsulated in the form of nanoemulsions.

中文翻译：

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不同储存温度下红麻种子水包油纳米乳的生物活性化合物稳定性和抗氧化活性

由酪蛋白酸钠 (SC)、β-环糊精 (β-CD) 和吐温 20 (T20) 稳定的红麻种子水包油纳米乳液 (NANO) 已被优化，并在之前的研究中显示可提高体外生物可及性和物理化学稳定性学习。本研究的主要目的是评估纳米颗粒在不同温度（4°C、25°C 和 40°C）下储存期间生物活性化合物和抗氧化剂的稳定性。对每种乳化剂的抗氧化活性的评价表明，SC具有良好的清除能力，具有97.6%的ABTS自由基清除活性。因此，SC作为主要乳化剂之一可以进一步增强NANO的抗氧化活性。在储存第 8 周时，储存在 4 °C 的 NANO 保持了最佳的生物活性化合物稳定性和抗氧化活性，其中维生素 E 的保留率为 90%，植物甾醇的保留率为 65%。这些结果表明，4°C 将是含有天然存在的维生素 E 和植物甾醇的 NANO 最合适的储存温度。从 40°C 的加速储存结果来看，含有维生素 E 和植物甾醇的 NANO 在储存的第 4 周和第 2 周之前一直保持其初始浓度的一半，这分别相当于在室温下储存 16 周和 8 周。实际应用 本研究的结果提供了对生物活性化合物的稳定性和抗氧化活性在水包油纳米乳液中的更好理解，该乳液在不同温度下储存期间由类似的三元乳化剂稳定。