Heparin, a highly sulfated linear polysaccharide, with anticoagulation function and blood compatibility is widely used as a biomaterials in medical application, but the most importance of heparin is its structure function as the macromolecular space arm. In this study, heparin as a spacer was covalently immobilized on the chloromethylated polystyrene microspheres (Ps) and then connected with L-phenylalanine forming the Ps-Hep-Phe structure, which was developed for endotoxin adsorption in hemoperfusion. The grafting density of heparin reach the maximum when the initial concentration of heparin solution was 5mg/mL. The adsorbents with the heparin as a spacer showed the prolonged clotting times, low protein adsorption, and reduced the hemolysis rate, indicating that heparin-modified adsorbents have great blood compatibility. The adsorption capacity of Ps-Hep-Phe for endotoxin was 25.15EU/g in dynamic adsorption, higher than that of Ps. Therefore, this study imply that heparin would be promising for modification of adsorbents in hemoperfusion.

肝素是一种高度硫酸化的线性多糖，具有抗凝功能和血液相容性，在医学应用中被广泛用作生物材料，但是肝素最重要的是其作为大分子空间臂的结构功能。在这项研究中，肝素作为间隔物被共价固定在氯甲基化的聚苯乙烯微球（Ps）上，然后与L-苯丙氨酸连接形成Ps- Hep-Phe结构，专为血液灌流中的内毒素吸附而开发。当肝素溶液的初始浓度为5mg / mL时，肝素的接枝密度达到最大值。以肝素为间隔物的吸附剂显示出延长的凝血时间，较低的蛋白质吸附和降低的溶血速率，表明肝素修饰的吸附剂具有良好的血液相容性。动态吸附中Ps-Hep-Phe对内毒素的吸附能力为25.15EU / g，高于Ps。因此，这项研究表明肝素有望在血液灌流中修饰吸附剂。