A series of novel N¹-(4,5-diazafluoren-9-yliden)-N²-glycopyranosyl hydrazines was prepared in synthetically useful yields by treatment of 9H-4,5-diazafluoren-9-hydrazone with different unprotected monosaccharides. The reactions with the monosaccharides tested afforded stereoselectively, and exclusively, cyclic derivatives, whose structures correspond to N-β-glycopyranosyl hydrazones except for the d-arabinose derivative that agrees with the α-anomer. Several copper(II) complexes having a 2:1 ligand to metal mole ratio were also prepared. The metal complexes can bind DNA sequences and preferentially stabilize G-quadruplex DNA structures over dsDNA. The fucose, rhamnose and deoxyglucose copper(II) complexes exhibited a cytotoxic activity against cultured HeLa and PC3 tumor cells comparable to other metal complexes normally used for chemotherapeutic purposes, such as cisplatin.

通过用不同的未保护的单糖处理9 H -4,5-二氮杂芴基-9- in，以合成有用的产率制备了一系列新颖的N ^1-（4，5-二氮杂芴-9-li基）-N ^2-甘氨酰呋喃糖基肼。与测试的单糖的反应可立体选择性地提供环状衍生物，其结构对应于N -β-甘露糖基，除了d-与α-端基异构体一致的阿拉伯糖衍生物。还制备了几种配体与金属的摩尔比为2：1的铜（II）配合物。金属配合物可以结合DNA序列，并且比dsDNA优先稳定G-四链体DNA结构。岩藻糖，鼠李糖和脱氧葡萄糖铜（II）配合物对培养的HeLa和PC3肿瘤细胞显示出与通常用于化学治疗目的的其他金属配合物（如顺铂）相当的细胞毒活性。