Abstract Rapid screening techniques for lignocellulosic biomass are required for the development of efficient conversion strategies in the field of biofuels and value-added chemicals. This is particularly true for tannins, which are highly valuable in the tannery and nutraceutical industries. In the present work, we propose a quick method based on reactive pyrolysis with in situ hexamethyldisilazane (HMDS) derivatization followed by GC/MS analysis for the qualitative determination of tannins in lignocellulosic biomass. Different pyrolysis times were used to study pyrolysis mechanisms of tannins. Reference compounds belonging to hydrolysable and condensed tannins, both in monomeric and polymeric forms, were used to select specific pyrolysis products that could be used as markers. When pyrolysis time was increased, hydrolysable tannins were found to be highly thermostable, while condensed tannins showed extensive degradation. An optimal pyrolysis time was determined to reduce the number of peaks and increase the abundances of the most characteristic components. The results were applied to assess the presence of tannins in four different biomass: oak gall nuts, hazelnut cuticles, grape seeds and pomegranate bark.

中文翻译：

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使用反应性 Py-GC/MS 和原位硅烷化快速筛选木质纤维素生物质中的可水解和缩合单宁

摘要 木质纤维素生物质的快速筛选技术需要在生物燃料和增值化学品领域开发有效的转化策略。单宁尤其如此，单宁在制革和保健品行业中非常有价值。在目前的工作中，我们提出了一种基于反应热解和原位六甲基二硅氮烷 (HMDS) 衍生化的快速方法，然后进行 GC/MS 分析，用于木质纤维素生物质中单宁的定性测定。不同的热解时间被用来研究单宁的热解机制。属于可水解和缩合单宁的参考化合物，无论是单体形式还是聚合形式，都用于选择可用作标记的特定热解产物。当热解时间增加时，发现可水解单宁具有高度的热稳定性，而缩合单宁则表现出广泛的降解。确定了最佳热解时间以减少峰的数量并增加最具特征成分的丰度。结果用于评估四种不同生物质中单宁的存在：橡木五倍子、榛子表皮、葡萄籽和石榴皮。