Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes,ACS Synthetic Biology

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Control of Lineage-Specific Gene Expression by Functionalized gRNA Barcodes
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2018-08-31 00:00:00 , DOI: 10.1021/acssynbio.8b00105
Aziz M Al'Khafaji _{1,

2} , Daniel Deatherage ₂ , Amy Brock _{1,

2}

Affiliation

Lineage tracking delivers essential quantitative insight into dynamic, probabilistic cellular processes, such as somatic tumor evolution and differentiation. Methods for high diversity lineage quantitation rely on sequencing a population of DNA barcodes. However, manipulation of specific individual lineages is not possible with this approach. To address this challenge, we developed a functionalized lineage tracing tool, Control of Lineages by Barcode Enabled Recombinant Transcription (COLBERT), that enables high diversity lineage tracing and lineage-specific manipulation of gene expression. This modular platform utilizes expressed barcode gRNAs to both track cell lineages and direct lineage-specific gene expression.

中文翻译：

通过功能化 gRNA 条形码控制谱系特异性基因表达

谱系追踪提供了对动态、概率性细胞过程（例如体细胞肿瘤进化和分化）的重要定量洞察。高多样性谱系定量方法依赖于对 DNA 条形码群体进行测序。然而，用这种方法不可能操纵特定的个体谱系。为了应对这一挑战，我们开发了一种功能化的谱系追踪工具，通过条形码重组转录控制谱系（COLBERT），它能够实现高多样性谱系追踪和谱系特异性的基因表达操作。该模块化平台利用表达的条形码 gRNA 来跟踪细胞谱系并指导谱系特异性基因表达。

更新日期：2018-08-31

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