A properly designed porous scaffold can accelerate the osseointegration process, and the use of computer-aided design (CAD) and additive manufacturing (AM) techniques has the potential to improve the traditional porous scaffold approach. In this study, we evaluate the effect of porous Ti₆Al₄V (Ti) with different pore structures on osteointegration and osteogenesis. Porous Ti scaffolds with different pore structures based on four commercially available implants were designed and manufactured by CAD and selective laser melting (SLM). Micro-CT showed that SLM was able to produce Ti scaffolds with different pore structures. The mechanical properties evaluated by finite element analysis and compression tests indicated that the four porous scaffolds in our study were mechanically adapted, despite their different mechanical properties. Then, we used 3D-printed porous discs to culture human bone marrow mesenchymal stem cells (hBMMSCs), the main seed cells of bone tissue engineering. The results showed no significant difference among the four groups in cell morphology, viability and proliferation. In addition, four groups showed a comparable mineralization ability even though Ti-g had a higher alkaline phosphatase activity (ALP). In vivo tests in a rabbit model showed that all four groups were suitable for new bone ingrowth and integration. These findings indicate that the four different pore structures in the Ti scaffolds provided good osteointegration and osteogenesis.

适当设计的多孔支架可以加速骨整合过程，并且计算机辅助设计（CAD）和增材制造（AM）技术的使用具有改进传统多孔支架方法的潜力。在这项研究中，我们评估了多孔Ti ₆ Al _4的作用V（Ti）具有不同的孔结构对骨整合和成骨作用。通过CAD和选择性激光熔融（SLM）设计和制造了基于四种可购买到的植入物的具有不同孔结构的多孔Ti支架。Micro-CT表明SLM能够生产具有不同孔结构的Ti支架。通过有限元分析和压缩测试评估的机械性能表明，我们研究中的四个多孔支架尽管具有不同的机械性能，但仍具有机械适应性。然后，我们使用3D打印的多孔圆盘培养人骨髓间充质干细胞（hBMMSC），这是骨组织工程的主要种子细胞。结果显示这四组在细胞形态，存活力和增殖方面没有显着差异。此外，在兔模型中进行的体内测试表明，所有四个组均适合新的骨长入和融合。这些发现表明，Ti支架中的四种不同的孔结构提供了良好的骨整合和成骨作用。