We developed a highly efficient method for patterning cells by a novel and simple technique called lift-off cell lithography (LCL). Our approach borrows the key concept of lift-off lithography from microfabrication and utilizes a fully biocompatible process to achieve high-throughput, high-efficiency cell patterning with nearly zero background defects across a large surface area. Using LCL, we reproducibly achieved >70% patterning efficiency for both adherent and non-adherent cells with <1% defects in undesired areas.

中文翻译：

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剥离式电池光刻技术，可在干净的背景下进行电池构图†

我们开发了一种高效的方法，通过一种称为剥离细胞光刻（LCL）的新颖且简单的技术来对细胞进行构图。我们的方法借鉴了微细加工的剥离光刻技术的关键概念，并利用完全生物相容的工艺来实现高通量，高效率的电池图案化，并在大表面积上具有几乎为零的背景缺陷。使用LCL，我们可以对粘附细胞和非粘附细胞的可重复性图案形成效率均达到> 70％，而在不良区域中的缺陷<1％。